01 May 2021>: Lab/In Vitro Research
Thrombin Aggravates Hypoxia/Reoxygenation Injury of Cardiomyocytes by Activating an Autophagy Pathway-Mediated by SIRT1Xiaoning Wang 1A , Yunhe Xu 2BC , Lingbo Li 3F , Weiwei Lu 1EF*
Med Sci Monit 2021; 27:e928480
Figure 3 Silencing of SIRT1 inhibited the effect of thrombin on the secretion of inflammatory factors and oxidative stress in H/R-injured cardiomyocytes. (A) MDA content, ROS level, and SOD activity were detected to evaluate oxidative stress in an H/R model treated with thrombin, an H/R model transfected with si-SIRT1 and dabigatran, an H/R model treated with a combination of thrombin and 3-MA, and controls. (B) ELISA was used to determine the level of IL-1b, IL-6, TNF-a, ICAM-1, and MMP-9 in serum. (C) MTT was conducted to test cell viability. *** P<0.001 vs control group, n=3.