01 May 2021>: Lab/In Vitro Research
Thrombin Aggravates Hypoxia/Reoxygenation Injury of Cardiomyocytes by Activating an Autophagy Pathway-Mediated by SIRT1Xiaoning Wang 1A , Yunhe Xu 2BC , Lingbo Li 3F , Weiwei Lu 1EF*
Med Sci Monit 2021; 27:e928480
Figure 4 Silencing of SIRT1 suppressed the regulation by thrombin of autophagy in H/R-injured astrocytes. (A) Western blotting was applied to determine the level of thrombin, PAR-1, SIRT1, Beclin1, and the conversion of LC3I to LC3II. (B) qRT-PCR was conducted to detect the expression of thrombin, PAR-1, and SIRT1. *** P<0.001 vs control group, n=3.