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27 January 2021: Lab/In Vitro Research

Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes

Qingxin Fan 12BCDEF , Pengjiao Xi 3ACD , Derun Tian 13AG , Lianqun Jia 4AFG , Yuan Cao 4BDF , Kaixuan Zhan 4BCDF , Tianwei Sun 5BCDG , Yinlong Zhang 6BCD , Qiming Wang 14ABCDEFG*

DOI: 10.12659/MSM.928619

Med Sci Monit 2021; 27:e928619

Figure 5 Effect of ginsenoside Rb1 (G-Rb1) on browning treated with SLK2001. (A) Oil Red O staining (ORS) detected the lipid droplets. Scale bar=100 μm. (B) Optical density values at 490 nm. (C) Lipid droplets’ diameter of ORS was measured by ImageJ. (D) Western blotting results of Ucp-1 and β-catenin, with Gapdh as the internal reference. (E, F) ImageJ quantized the gray value of each protein band. (G) Immunofluorescence was performed to detect the expression of Ucp-1. Scale bar=50 μm. Data are expressed as mean±SD (n=3). * P<0.05, ** P<0.01, **** P<0.0001.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750