27 January 2021 : Laboratory Research
Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes
Qingxin Fan12BCDEF, Pengjiao Xi3ACD, Derun Tian13AG, Lianqun Jia4AFG, Yuan Cao4BDF, Kaixuan Zhan4BCDF, Tianwei Sun5BCDG, Yinlong Zhang6BCD, Qiming Wang14ABCDEFG*DOI: 10.12659/MSM.928619
Med Sci Monit 2021; 27:e928619
Figure 5 Effect of ginsenoside Rb1 (G-Rb1) on browning treated with SLK2001. (A) Oil Red O staining (ORS) detected the lipid droplets. Scale bar=100 μm. (B) Optical density values at 490 nm. (C) Lipid droplets’ diameter of ORS was measured by ImageJ. (D) Western blotting results of Ucp-1 and β-catenin, with Gapdh as the internal reference. (E, F) ImageJ quantized the gray value of each protein band. (G) Immunofluorescence was performed to detect the expression of Ucp-1. Scale bar=50 μm. Data are expressed as mean±SD (n=3). * P<0.05, ** P<0.01, **** P<0.0001.