Figure 1 SPOP knockout promotes C4-2 cell migration and invasion. (A) Photomicrograph of sgSPOP-1 and sgSPOP-2 compared with parent cell line C4-2 (control). (B) Western blotting with anti-SPOP antibody confirms the presence of SPOP in control C4-2 cells and absence in both sgSPOP-1 and sgSPOP-2 cells. (C) A transwell assay was used to explore cell migration and invasion ability in sgSPOP-1, sgSPOP-2, and control cells. The results are presented as the mean±SD of 3 independent replicates; * P<0.05, ** P<0.01. (D) The migration ability of sgSPOP-1 cells, sgSPOP-2 cells, and control cells was investigated using a scratch wound-healing assay. The results are shown as the mean±SD of 3 independent replicates; * P<0.05, ** P<0.01.