08 December 2021 : Laboratory Research
Chemokine (C-C Motif) Ligand 2/Chemokine Receptor 2 (CCR2) Axis Blockade to Delay Chondrocyte Hypertrophy as a Therapeutic Strategy for Osteoarthritis
Zidong Wang1ACE, Bei Wang2CF, Jian Zhang1DF, Zhensong Wu3BD, Liankui Yu1BF, Zhongye Sun1ADG*DOI: 10.12659/MSM.930053
Med Sci Monit 2021; 27:e930053
Figure 2 CCR2 deficiency delays chemokine (C-C motif) ligand 2 (CCL2)-induced chondrocyte hypertrophy (CH). (A) Protein expression of CCL2 and CCR2 was analyzed after treatment with bindarit or CAS and quantified. mRNA expression of (B) CCL2 and (C) CCR2 was analyzed after treatment with CCL2 protein with or without CAS pretreatment. (D) Expression of Type 2 collagen, RUNX2, and IHH mRNA in CH. (E) Cell proliferation was measured with an MTT assay. (F) Cell apoptosis was measured with flow cytometry. Data are shown as means±standard deviations vs the control group. * P<0.05, ** P<0.01, *** P<0.001.