08 December 2021: Lab/In Vitro Research
Chemokine (C-C Motif) Ligand 2/Chemokine Receptor 2 (CCR2) Axis Blockade to Delay Chondrocyte Hypertrophy as a Therapeutic Strategy for Osteoarthritis
Zidong Wang 1ACE , Bei Wang 2CF , Jian Zhang 1DF , Zhensong Wu 3BD , Liankui Yu 1BF , Zhongye Sun 1ADG*DOI: 10.12659/MSM.930053
Med Sci Monit 2021; 27:e930053
Figure 3 CCR2 deficiency inhibits chemokine (C-C motif) ligand 2 (CCL2)-induced Type 10 collagen expression. (A) Representative images of cell morphology and (B) cell area measurement. (C) Protein expression of Type 10 collagen and CCL2 was determined with immunofluorescence (IF) (magnification: 200×). Red indicates Type 10 collagen, green indicates CCL2, and blue indicates the cell nucleus. (D) Quantification analysis of IF intensity. (E) Protein expression of Type 10 collagen, CCL2, and CCR2 and (F) quantification analysis. Data are shown as means±standard deviations vs the control group. * P<0.05, *** P<0.001.