08 December 2021 : Laboratory Research
Chemokine (C-C Motif) Ligand 2/Chemokine Receptor 2 (CCR2) Axis Blockade to Delay Chondrocyte Hypertrophy as a Therapeutic Strategy for Osteoarthritis
Zidong Wang1ACE, Bei Wang2CF, Jian Zhang1DF, Zhensong Wu3BD, Liankui Yu1BF, Zhongye Sun1ADG*DOI: 10.12659/MSM.930053
Med Sci Monit 2021; 27:e930053
Figure 4 Blocking chemokine (C-C motif) ligand 2 (CCL2)/CCR2 inhibits interleukin-17A-induced Type 10 collagen expression. (A) Representative images of cell morphology and (B) cell area measurement. (C) Protein expression of Type 10 collagen and CCL2 was determined with immunofluorescence (IF) (magnification: 200×). Red indicates Type 10 collagen, green indicates CCL2, and blue indicates the cell nucleus. (D) Quantification analysis of IF intensity. (E) Protein expression of Type 10 collagen, CCL2, and CCR2 and (F) quantification analysis. Data are shown as means±standard deviations vs the control group. * P<0.05, *** P<0.001.