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03 April 2021: Animal Study

Expression of DUSP12 Reduces Lung Vascular Endothelial Cell Damage in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury via the Apoptosis Signal-Regulating Kinase 1 (ASK1)-Jun N-Terminal Kinase Activation (JNK) Pathway

Zhao Hui 1BF , Huang Jie 2CD , Guo-Hua Fan 2ABCDEFG*

DOI: 10.12659/MSM.930429

Med Sci Monit 2021; 27:e930429

Figure 7 ASK1-JNK inhibition alleviated the effects of DUSP12 silencing. MLECs were transfected with DUSP12 siRNA, treated with the JNK inhibitor or ASK1 siRNA and then treated with LPS. (A) ASK1 protein levels after cells were transfected with ASK1 siRNA. (B) ELISA measurement of proinflammatory factors. (C) ROS level, MDA level, NADPH activity, and SOD activity. (D) TUNEL staining (magnification: 200). (E) Caspase-3 activity (* P<0.05 vs ScRNA/LPS; # P<0.05 vs siDUSP12/LPS). All in vitro studies were repeated 3 times independently.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750