Figure 3 High glucose induced upregulation of integrin β6. HT-29 and WiDr cells were incubated with medium containing various concentrations of glucose for 24 h, and expression of integrin β6 and αv was measured by real-time PCR (A, B) and western blot (C, D). (E) Expression of integrin β1, β3, and β5 was detected by real-time PCR in HT-29 cells. Mean±SD of 3 independent experiments. * P<0.05 vs control. OC – osmotic control; NG – normal glucose; HG – high glucose.