Figure 4 The ERK pathway was involved in high-glucose-induced upregulation of integrin αvβ6. (A, B) HT-29 and WiDr cells were incubated with normal glucose, high-glucose, and osmotic control medium, and expression of total-ERK (t-ERK) and phospho-ERK (p-ERK) was determined by western blot. (C–E) HT-29 and WiDr cells were pretreated with ERK-specific inhibitor U0126 for 30 min followed by stimulation with high-glucose medium for 24 h, and protein (C, D) and mRNA (E) expression of β6 subunit was detected by western blot and real-time PCR. (F) Cell surface expression of integrin αvβ6 was detected by flow cytometry in HT-29 cells. * P<0.05 vs NG. # P<0.05 vs HG. NG – normal glucose; HG – high glucose.