Figure 6 High glucose increased mRNA expression and activity of MMP-9. HT-29 cells were transfected with β6 siRNA or negative control for 24 h, and cells were incubated in normal glucose, high-glucose, or osmotic control medium. (A, B) mRNA expression of metalloproteinase-9 (MMP-9) and metalloproteinase-2 (MMP-2) was measured by real-time PCR. (C–E) Cell supernatant was collected, and the activity of MMP-9 and MMP-2 was determined by gelatin zymography. * P<0.05 vs NG. # P<0.05 vs HG.