20 August 2021 : Animal Research
Knockout of the Cannabinoid Receptor 2 Gene Promotes Inflammation and Hepatic Stellate Cell Activation by Promoting A20/Nuclear Factor-κB (NF-κB) Expression in Mice with Carbon Tetrachloride-Induced Liver Fibrosis
Cuizhen Long123ABCDEF, Na Xie12ABCDEF, Yuanhui Shu12ABCF, Yafeng Wu14ABCF, Ping He12BC, Yan Zhou12BC, Yining Xiang5CD, Junying Gu12CD, Lei Yang12DF, Yuping Wang12ABCDEFG*DOI: 10.12659/MSM.931236
Med Sci Monit 2021; 27:e931236
Figure 1 Identification of cannabinoid receptor 2 gene knockout. The extracted DNA from the tails of mice was subjected to PCR reaction and agarose gel electrophoresis, and the following results were obtained: the DNA band of CB2−/− mice (A, B, C, D, E, F) was about 550 bp, and the DNA band of WT mice (I) was about 385 bp. The negative control group (G) did not have any product bands, and the hybrid mice (H) had 2 bands (550 bp and 385 bp).