10 January 2022>: Clinical Research
Expression of Long Non-Coding RNA (lncRNA) SNHG5 in Patients with Refractory Diabetic Macular Edema and Its Regulatory Mechanism
Junwen He 1ABEFG* , Zhang Rui 1BC , Jing Gao 2DE , Yanhong Chen 2DEF , Yanzi Li 1BCD , Tao Xu 1BDF , Shan Wang 2ABCE*DOI: 10.12659/MSM.932996
Med Sci Monit 2022; 28:e932996
Figure 6 SNHG5 reversed the effect of high glucose induction. RT-qPCR (A) analysis showed that the expression of SNHG5 was different in HRMECs induced by high glucose. (B) RT-qPCR analyzed the expression of SNHG5 after transfecting HRMECs with the plasmid overexpressing lncRNA SNHG5 and 3 si-SNHG5. After transfection of ov-SNHG5 or si-SNHG5, RT-qPCR (C) assays and CCK8 (D) assays were performed to analyze the changes in expression of SNHG5 and cell proliferation in each group. Transfection of ov-SNHG5 or si-SNHG5 was divided into the control group, HG group, HG+ov-NC group, HG+ov-SNHG5 group, HG+si-NC group, and HG+si-SNHG5 group. * P<0.05; CCK8 – Cell Counting Kit-8; HG – high glucose; NC – negative control; HRMECs – Human Retinal Microvascular Endothelial Cells. Photoshop software (version 21.2.4; Adobe, Inc; San Jose, USA) was used for creation of the figure.