07 March 2022: Animal Study
Astragalus Flavone Induces Proliferation and Differentiation of Neural Stem Cells in a Cerebral Infarction Model
Han Gao CEF* , Ningjing Huang BE* , Weiwei Wang BC , Lingling Zhang CD , Li Cai D , Min Chen F , Wentao Li AG*DOI: 10.12659/MSM.933830
Med Sci Monit 2022; 28:e933830
Figure 5 ASF induced NSCs proliferation in vitro (A) The bright field image of neurospheres after culturing for 3 days (A1, Scale bar: 200 μm), and the representative IF image of Nestin confirmed that these derived cells were NSCs (A2, Scale bar: 100 μm). (B) Representative IF images of EdU+ cells treated with ASF at concentrations of 0, 10, 20 and 40 μg/mL. DAPI was used to counterstain nuclei. Scale bar: 100μm. (C) The number of EdU+ cells. Data are presented as mean±SEM, * P<0.05, ** P<0.01.