Figure 2 Representative EEG recordings from hippocampal electrodes (Omniplex Neural Data Acquisition System, Plexon, Inc.) in control, 10 min, and 30 min after SE onset with diazepam therapy. SE was induced via an injection of lithium chloride (5 mEq/kg, i.p.) and pilocarpine hydrochloride (40 mg/kg, i.p.) 16 h later. Rats in each time point group were treated with the same volume of placebo (control, n=5) or 10 mg/kg diazepam (n=8) after 5 min, 10 min, 20 min, and 30 min of SE. As illustrated in the post-treatment traces, treatments at 5 min or 10 min initially eliminated the epileptiform discharges. As a result, behavioral seizures were terminated as well. However, 10 mg/kg diazepam monotherapy, when SE lasted more than 10 min, failed to decrease EEG power to reach twice the pre-seizure baseline without bursting. Thus, each trace represents a 20-s period. Scale bar (upper left), 800 μV. EEG, electroencephalograph; SE, status epilepticus.