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10 May 2022: Lab/In Vitro Research

Impact of Factors Secreted by Tumor Cells on Response of Pleural Mesothelial Cells to Different Sclerosing Agents in an In Vitro Model

Michał Mierzejewski ABEF , Magdalena Paplińska-Goryca ABCDE* , Rafał Krenke ADEG

DOI: 10.12659/MSM.936065

Med Sci Monit 2022; 28:e936065

Figure 1 A flow chart presenting the general study design, including phase 1 and 2. Initially, adenocarcinoma (ADC) cells were cultured and supernatant was collected. After that, mesothelial cell cultures where incubated with or without supernatant from ADC and treated or not treated with a sclerosant. After 24 h, the levels of cytokines in cell culture supernatants and mRNA in cell lysates were measured (end of phase 1). Then, supernatants from mesothelial cell cultures were used to stimulate fibroblast culture (phase 2). The fibroblast culture was assessed for collagen secretion. The whole experiment was repeated for each of the following sclerosants: talc (20 μg/mL), PVP-I (0.001%), doxycycline (2 μg/mL), and TGF-β (0.2 μg/mL). Created with BioRender.com.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750