24 June 2020 : Clinical Research
Comprehensive Analysis of Candidate Diagnostic and Prognostic Biomarkers Associated with Lung Adenocarcinoma
Jingyuan Li1AE, Xingyuan Liu23CF, Zan Cui1B, Guanying Han1DG*DOI: 10.12659/MSM.922070
Med Sci Monit 2020; 26:e922070
Table 3 Primer pair sequences for RT-qPCR of PECAM1, CDK1, MKI67, SPP1, TOP2A, CHEK1, CCNB1, RRM2, and GAPDH.
| Gene | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
| PECAM1 | atgccagtggaaatgtcc | tcagaagtggtactggtg |
| CDK1 | ccgtcgtaacctgttgagtaactat | gtctacccttatacaccacaccgtaa |
| MKI67 | cgcgaattcagagagcttttccagacaccatg | cgagcctcgaggaagattgttggggtacccac |
| SPP1 | ttctgattgggacagccgtg | tctcatcattggctttccgct |
| TOP2A | ggtgagaaggactggcagaaat | cttgtcgatgaagtacagggcta |
| CHEK1 | ccagatgctcagagattcttcca | tgttcaacaaacgctcacgatta |
| CCNB1 | ttcgcctgagcctattttggta | agctccatcttctgcatccacat |
| RRM2 | tttagtgagcttagcacagcggga | aaatctgcgttgaagcagtgaggc |
| GAPDH | tccaccaccctgttgctgta | gacttcaacagcaactcccac |