18 October 2020 : Animal Research
Silencing of Long Non-Coding RNA X Inactive Specific Transcript (Xist) Contributes to Suppression of Bronchopulmonary Dysplasia Induced by Hyperoxia in Newborn Mice via microRNA-101-3p and the transforming growth factor-beta 1 (TGF-β1)/Smad3 Axis
Wenhao Yuan1ABCDEF, Xiaoyan Liu1ABCDG, Lingkong Zeng1BCDFG*, Hanchu Liu1ABCDEFG, Baohuan Cai2ABCDEFG, Yanping Huang1ABDF, Xuwei Tao1ABCDF, Luxia Mo1ABDF, Lingxia Zhao1BCDF, Chunfang Gao1BCDFDOI: 10.12659/MSM.922424
Med Sci Monit 2020; 26:e922424
Table 1 Primer sequences of RT-qPCR.
| Gene | Sequence (5′-3′) |
|---|---|
| miR-101-3p | Forward primer: 5′-UACAGUACUGUGAUAACUGAA-3′ |
| Reverse primer: 5′-CAGUUAUCACAGUACUGUAUU-3′ | |
| U6 | Forward primer: 5′-GCUUCGGCAGCACAUAUACUAAAAU-3′ |
| Reverse primer: ′-CGCUUCACGAAUUUGCGU GUCAU-3′ | |
| Xist | Forward primer: 5′-ACGCTGCATGTGTCCTTAG-3′ |
| Reverse primer: 5′-GAGCCTCTTATAGCTGTTTG-3′ | |
| VEGF | Forward primer: 5′-TTCATGGACGTCTACCAGCG- 3′ |
| Reverse primer: 5′-CTTCATCATTGCAGCAGCCC- 3′ | |
| Collagen I | Forward primer: 5′-GTGCTAAAGGTGCCAATGGT-3′ |
| Reverse primer: 5′-ACCAGGTTCACCGCTGTTAC-3′ | |
| α-SMA | Forward primer: 5′-CTTGCTAACGGAGGCG-3′ |
| Reverse primer: 5′-TCCAGAGTCCAGCACAATA-3′ | |
| HMGB3 | Forward primer: 5′-ATTCGGAATTCCGTATCTGGCCTTTTGAC-3′ |
| Reverse primer: 5′-CGGTTACTCGGCTTACGCTTGGACTG-3′ | |
| GAPDH | Forward primer: 5′-GTCAACGGATTTGGTCTGTATT-3′ |
| Reverse primer: 5′-AGTCTTCTGGGTGGCAGTGAT-3′ | |
| RT-qPCR – reverse transcription-quantitative polymerase chain reaction; miR – microRNA; Xist – X inactive specific transcript; VEGF – vascular endothelial growth factor; α-SMA – alpha-smooth muscle Actin; HMGB3 – high-mobility group protein 3; GAPDH – glyceraldehyde-3-phosphate dehydrogenase. |