25 August 2020: Lab/In Vitro Research
Dioscin Attenuates Interleukin 1β (IL-1β)-Induced Catabolism and Apoptosis via Modulating the Toll-Like Receptor 4 (TLR4)/Nuclear Factor kappa B (NF-κB) Signaling in Human Nucleus Pulposus Cells
Longhui Wang DEF , Yuntao Gu BE , Hai Zhao CEF , Rong Chen BD , Wensheng Chen BF , Hao Qi BE , Weisong Gao ABG*DOI: 10.12659/MSM.923386
Med Sci Monit 2020; 26:e923386
Table 1 Primers of targeted genes.
| Forward | Reverse | |
|---|---|---|
| MMP3 | CTGGCCTGCTGGCTCATGCTT | GCAGGGTCCTTGGAGTGGTCA |
| MMP13 | AGCCACTTTATGCTTCCTGATG | GATGTTTAGGGTTGGGGTCTTC |
| ADAMTS4 | CCTGGCAAGGACTATGATGCTGA | GGGCGAGTGTTTGGTCTGG |
| ADAMTS5 | CGCTCTCTGGCTGTCTTTGAC | AGCAGTGGCTTTAGGGTGTAGC |
| Collagen II | CTCAAGTCGCTGAACAACCA | GTCTCCGCTCTTCCACTCTG |
| Aggrecan | GTCAGATACCCCATCCACACTC | CATAAAAGACCTCACCCTCCAT |
| IL-6 | ATGAACTCCTTCTCCACAAGC | CTACATTTGCCGAAGAGCCCTCAGGCTGGACTG |
| TNF-α | AGGCGGTGCTTGTTCCTCA | GTTCGAGAAGATGATCTGACTGCC |
| β-actin | ATTGCCGACAGGATGCAGAA | GCTGATCCACATCTGCTGGA |