02 July 2020 : Clinical Research
Nuclear Factor κB/MicroRNA-155 Upregulates the Expression Pattern of Cytokines in Regulating the Relapse of Chronic Sinusitis with Nasal Polyps and the Underlying Mechanism of Glucocorticoid
Jianbin Du1BCE, Haijun Lv2BC, Xin Dou3BC, Zhongsheng Cao1ADEF*DOI: 10.12659/MSM.923618
Med Sci Monit 2020; 26:e923618
Table 2 Sequences of RNA primers.
| U6 for human | RT: 5′-CGCTTCACGAATTTGCGTGTCAT-3′ |
| F: 5′-GCTTCGGCAGCACATATACTAAAAT-3′ | |
| R: 5′-CGCTTCACGAATTTGCGTGTCAT-3′ | |
| miR-155 for human | RT: 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCCCTA-3′ |
| F: 5′-GGAGGTTAATGCTAATCGTGATAG-3′ | |
| R: 5′-GTGCAGGGTCCGAGGT-3′ | |
| U6 for mice | RT: 5′-AACGCTTCACGA ATTTGCGT-3′ |
| F: 5′-CTCGCTTCGGCAGCACA-3′ | |
| R: 5′-AACGCTTCACGA ATTTGCGT-3′ | |
| miR-155 for mice | RT: 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCCCTA-3′ |
| F: 5′-CTCGTGGTTAATGCTAATTGTGA-3′ | |
| R: 5′-GTGCAGGGTCCGAGGT-3′ | |
| TNF-α for human | F: 5′-GCCTCTTCTCCTTCCTGAT-3′ |
| R: 5′-AGATGATCTGACTGCCTGG-3′ | |
| TNF-α for mice | F: 5′-CGTGGAACTGGCAGAAGA-3′ |
| R: 5′-GTAGACAGAAGAGCGTGGT-3′ | |
| IL-1 for human | F: 5′-GACAGGATATGGAGCAACAAG-3′ |
| R: 5′-CAACACGCAGGACAGGTA-3′ | |
| IL-1 for mice | F: 5′-TGACAGTGATGAGAATGACCT-3′ |
| R: 5′-ATGTGCTGCTGCGAGATT-3′ | |
| IL-4 for human | F: 5′-CAGTTCCACAGGCACAAG-3′ |
| R: 5′-TGGTTGGCTTCCTTCACA-3′ | |
| IL-4 for mice | F: 5′-TGCTTGAAGAAGAACTCTAGTG-3′ |
| R: 5′-GATGTGGACTTGGACTCATTC-3′ | |
| IL-5 for human | F: 5′-AGGCACACTGGAGAGTCA-3′ |
| R: 5′-GCAGGTAGTCTAGGAATTGGT-3′ | |
| IL-5 for mice | F: 5′-GACAAGCAATGAGACGATGA-3′ |
| R: 5′-CCCACGGACAGTTTGATTC-3′ | |
| β-actin for human | F: 5′-ATCAAGATCATTGCTCCTCCT-3′ |
| R: 5′-GACTCGTCATACTCCTGCTT-3′ | |
| β-actin for mice | F: 5′-GATTACTGCTCTGGCTCCTA-3′ |
| R: 5′-TACTCCTGCTTGCTGATCC-3′ | |
| TNF – tumor necrosis factor; IL – interleukin. |