02 July 2020: Clinical Research
Nuclear Factor κB/MicroRNA-155 Upregulates the Expression Pattern of Cytokines in Regulating the Relapse of Chronic Sinusitis with Nasal Polyps and the Underlying Mechanism of Glucocorticoid
Jianbin Du 1BCE , Haijun Lv 2BC , Xin Dou 3BC , Zhongsheng Cao 1ADEF*DOI: 10.12659/MSM.923618
Med Sci Monit 2020; 26:e923618
Table 2 Sequences of RNA primers.
| U6 for human | RT: 5′-CGCTTCACGAATTTGCGTGTCAT-3′ |
| F: 5′-GCTTCGGCAGCACATATACTAAAAT-3′ | |
| R: 5′-CGCTTCACGAATTTGCGTGTCAT-3′ | |
| miR-155 for human | RT: 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCCCTA-3′ |
| F: 5′-GGAGGTTAATGCTAATCGTGATAG-3′ | |
| R: 5′-GTGCAGGGTCCGAGGT-3′ | |
| U6 for mice | RT: 5′-AACGCTTCACGA ATTTGCGT-3′ |
| F: 5′-CTCGCTTCGGCAGCACA-3′ | |
| R: 5′-AACGCTTCACGA ATTTGCGT-3′ | |
| miR-155 for mice | RT: 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCCCTA-3′ |
| F: 5′-CTCGTGGTTAATGCTAATTGTGA-3′ | |
| R: 5′-GTGCAGGGTCCGAGGT-3′ | |
| TNF-α for human | F: 5′-GCCTCTTCTCCTTCCTGAT-3′ |
| R: 5′-AGATGATCTGACTGCCTGG-3′ | |
| TNF-α for mice | F: 5′-CGTGGAACTGGCAGAAGA-3′ |
| R: 5′-GTAGACAGAAGAGCGTGGT-3′ | |
| IL-1 for human | F: 5′-GACAGGATATGGAGCAACAAG-3′ |
| R: 5′-CAACACGCAGGACAGGTA-3′ | |
| IL-1 for mice | F: 5′-TGACAGTGATGAGAATGACCT-3′ |
| R: 5′-ATGTGCTGCTGCGAGATT-3′ | |
| IL-4 for human | F: 5′-CAGTTCCACAGGCACAAG-3′ |
| R: 5′-TGGTTGGCTTCCTTCACA-3′ | |
| IL-4 for mice | F: 5′-TGCTTGAAGAAGAACTCTAGTG-3′ |
| R: 5′-GATGTGGACTTGGACTCATTC-3′ | |
| IL-5 for human | F: 5′-AGGCACACTGGAGAGTCA-3′ |
| R: 5′-GCAGGTAGTCTAGGAATTGGT-3′ | |
| IL-5 for mice | F: 5′-GACAAGCAATGAGACGATGA-3′ |
| R: 5′-CCCACGGACAGTTTGATTC-3′ | |
| β-actin for human | F: 5′-ATCAAGATCATTGCTCCTCCT-3′ |
| R: 5′-GACTCGTCATACTCCTGCTT-3′ | |
| β-actin for mice | F: 5′-GATTACTGCTCTGGCTCCTA-3′ |
| R: 5′-TACTCCTGCTTGCTGATCC-3′ | |
| TNF – tumor necrosis factor; IL – interleukin. |