30 August 2020 : Laboratory Research
Honokiol Suppression of Human Epidermal Growth Factor Receptor 2 (HER2)-Positive Gastric Cancer Cell Biological Activity and Its Mechanism
Yidan Yan1ABC, Jianmin Xu2CDE, Guoxin Mao3EFG*DOI: 10.12659/MSM.923962
Med Sci Monit 2020; 26:e923962
Table 1 Relative primer sequences.
| Gene name | Primer sequence |
|---|---|
| HER2a | F: 5′-CCATCAAAGTGTTGAGGGAAAC-3′ |
| R: 5′-AATCTGCATACACCAGTTCAGCA-3′ | |
| PI3K | F: 5′-CATCACTTCCTCCTGCTCTAT-3′ |
| R: 5′-CAGTTGTTGGCAATCTTCTTC-3′ | |
| AKT | F: 5′-GGACAACCGCCATCCAGACT-3′ |
| R: 5′-GCCAGGGACACCTCCATCTC-3′ | |
| P21 | F: 5′-CCATCGATGGCAGCTCTTGCAGAAGCTC-3′ |
| R: 5′-GCTCTAGACTGGCCGGATTTGCGCGG-3′ | |
| MMP-9 | F: 5′-GAGACCGGTGAGCTGGATAG-3′ |
| R: 5′-TACACGCGAGTGAAGGTGAG-3′ | |
| GAPDH | F: 5′-GGTGAAGGTCGGAGTCAACG-3′ |
| R: 5′-CAAAGTTGTCATGGATGACC-3′ | |
| HER2 – human epidermal growth factor receptor 2; PI3K – phosphatidylinositol 3-kinase; AKT – protein kinase B; MMP – matrix metalloproteinase; GAPDH – glyceraldehyde-3-phosphate dehydrogenase; F – forward; R – reverse. |