27 June 2020 : Animal Research
Pyrroloquinoline Quinone Inhibits Oxidative Stress in Rats with Diabetic Nephropathy
Min Zhang12ACD, Jiangzhao Zhang3BE, Yan Xiong2CF, Jiaqing Peng2BF, Xiaoyan Wu1AEG*DOI: 10.12659/MSM.924372
Med Sci Monit 2020; 26:e924372
Table 1 Real time PCR primers.
| Gene name | Forward (5′>3′) | Reverse (5′>3′) |
|---|---|---|
| SOD1 | GGTGAACCAGTTGTGTTGTC | CCGTCCTTTCCAGCAGTC |
| SOD2 | CAGACCTGCCTTACGACTATGG | CTCGGTGGCGTTGAGATTGTT |
| GPX1 | ATCATATGTGTGCTGCTCGGCTAGC | TACTCGAGGGCACAGCTGGGCCCTTGAG |
| GPX3 | AGAGCCGGGGACAAGAGAA | ATTTGCCAGCATACTGCTTGA |
| AMPK | AAAGAACCCTAGCCTGAAGAGG | ACCTTCCGAGATGAATGCTTTT |
| FOXO3a | CTGGGGGAACCTGTCCTATG | TCATTCTGAACGCGCATGAAG |
| GAPDH | ACAACTTTGGTATCGTGGAAGG | GCCATCACGCCACAGTTTC |
| PCR – polymerase chain reaction; SOD – superoxide dismutase; GPX – glutathione peroxidase; AMPK – adenosine 5′-monophosphate-activated protein kinase; FOXO3a – forkhead box protein O3a; GAPDH – glyceraldehyde 3-phosphate dehydrogenase. | ||