11 August 2020>: Lab/In Vitro Research
Regulates the Proliferation and Migration of H9c2 Cells
Hongshu Wang 1BCDEF* , Yong Liu 2BC* , Shen Han 1B , Yunfeng Zi 1B , Yayong Zhang 1B , Ruize Kong 3B , Zu Liu 1B , Zhibin Cai 1B , Chongbin Zhong 4B , Wei Liu 5B , Lifeng Li 4B , Lihong Jiang 3AG*DOI: 10.12659/MSM.925388
Med Sci Monit 2020; 26:e925388
Table 2 The protocol of quantitative reverse transcription-polymerase chain reaction (qRT-PCR) of mRNAs.
Temperature | Times | Cycle | |
---|---|---|---|
Pre-denature | 95°C | 2 min | 1 cycle |
Amplification | 95°C | 15 s | 40 cycles |
60°C | 50 s | ||
Melting curves | 95°C | 5 s | 1 cycle |
65°C | 5 s | 1 cycle | |
95°C | Hold | 1 cycle |