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11 August 2020: Lab/In Vitro Research

Regulates the Proliferation and Migration of H9c2 Cells

Hongshu Wang 1BCDEF* , Yong Liu 2BC* , Shen Han 1B , Yunfeng Zi 1B , Yayong Zhang 1B , Ruize Kong 3B , Zu Liu 1B , Zhibin Cai 1B , Chongbin Zhong 4B , Wei Liu 5B , Lifeng Li 4B , Lihong Jiang 3AG*

DOI: 10.12659/MSM.925388

Med Sci Monit 2020; 26:e925388

Table 2 The protocol of quantitative reverse transcription-polymerase chain reaction (qRT-PCR) of mRNAs.

TemperatureTimesCycle
Pre-denature95°C2 min1 cycle
Amplification95°C15 s40 cycles
60°C50 s
Melting curves95°C5 s1 cycle
65°C5 s1 cycle
95°CHold1 cycle

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750