23 November 2020: Animal Study
Intermittent Fasting Improves Lipid Metabolism Through Changes in Gut Microbiota in Diet-Induced Obese Mice
Ya Deng 1BEF* , Wanjun Liu 1CE* , Jianqing Wang DF2* , Jun Yu 2D , Li-qi Yang 1AG*DOI: 10.12659/MSM.926789
Med Sci Monit 2020; 26:e926789
Table 1 Primers used for real-time polymerase chain reaction.
| Primer | Amplicon size (base pairs) | Forward primer (5′→3′) | Reverse primer (5′→3′) |
|---|---|---|---|
| β-actin | 120 | AGTGTGACGTTGACATCCGT | TGCTAGGAGCCAGAGCAGTA |
| Ucp1 | 191 | CACGGGGACCTACAATGCTT | ACAGTAAATGGCAGGGGACG |
| Fas | 96 | CTGCCTCTGGTGCTTGCT | TTCACGAACCCGCCTCCTC |
| Cd36 | 173 | GGCTGTGTTTGGAGGCATTC | CCACGTCATCTGGGTTTTGC |
| PPARγ | 128 | CTCACAATGCCATCAGGTTTGG | CAAATGCTTTGCCAGGGCTC |
| PPARα | 96 | ACCTTGTGTATGGCCGAGAA | CTGGCAGCAGTGGAAGAATC |