Effects of Diabetes Mellitus on Sperm Quality in the Db/Db Mouse Model and the Role of the FoxO1 Pathway

Jisheng Wang; Binghao Bao; Junlong Feng; Qi Zhao; Hengheng Dai; Fanchao Meng; Sheng Deng; Bin Wang; Haisong Li

doi:10.12659/MSM.928232

16 February 2021: Animal Study

Effects of Diabetes Mellitus on Sperm Quality in the Db/Db Mouse Model and the Role of the FoxO1 Pathway

Jisheng Wang ^12ABDE* , Binghao Bao ^12ABE* , Junlong Feng ^12ABCE* , Qi Zhao ^12BCF* , Hengheng Dai ^12BDF , Fanchao Meng ^12BF , Sheng Deng ^12DF , Bin Wang ^2AG* , Haisong Li ^2AE*

DOI: 10.12659/MSM.928232

Med Sci Monit 2021; 27:e928232

Authors information Article notes Copyright and License information

Background Material and Methods Results Discussion Conclusions References

View HTML version

Table 1 The primer sequences for qPCR.

Primer name		Primer pairs (5′to3′)
PI3K	Upstream prim	AATGATGCTTGGCTCTGGAATG
PI3K	Downstream prim	TGCTGCTTGATGGTGTGGAA
AKT	Upstream prim	ATGAACGACGTAGCCATTGTG
AKT	Downstream prim	TTGTAGCCAATAAAGGTGCCAT
Foxo1	Upstream prim	TACGCCGACCTCATCACCAA
Foxo1	Downstream prim	CACGCTCTTCACCATCCACTC
FasL	Upstream prim	TGGTTCTGGTGGCTCTGGTT
FasL	Downstream prim	GGTTGGCTCACGGAGTTCTG
Stat3	Upstream prim	TATGGTCCTTATTCTATGCG
Stat3	Downstream prim	CAGACAGTTGCCAGTCTCA
IL-6	Upstream prim	TAGTCCTTCCTACCCCAATTTCC
IL-6	Downstream prim	TTGGTCCTTAGCCACTCCTTC
β-actin	Upstream prim	GCCTTCCTTCTTGGGTAT
β-actin	Downstream prim	GGCATAGAGGTCTTTACGG

Back to the Article

Effects of Diabetes Mellitus on Sperm Quality in the Db/Db Mouse Model and the Role of the FoxO1 Pathway

Your Privacy