Zi Qi Decoction Alleviates Liver Fibrosis by Inhibiting the Toll-Like Receptor 4 (TLR4)-Related Nuclear Factor kappa b (NF-κB) and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways

Jingwen Zhou; Xiaolong Zhang; Lingfeng Wan; Jun Yu; Tianci Li; Ziyu Lu; Nanyuan Fang; Lixia Sun; Fang Ye

doi:10.12659/MSM.929438

14 April 2021 : Animal Research

Zi Qi Decoction Alleviates Liver Fibrosis by Inhibiting the Toll-Like Receptor 4 (TLR4)-Related Nuclear Factor kappa b (NF-κB) and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways

Jingwen Zhou^1ABCE, Xiaolong Zhang^1A, Lingfeng Wan^1AD, Jun Yu^2BCD, Tianci Li^2BCD, Ziyu Lu^3BCD, Nanyuan Fang^1E, Lixia Sun^1AG, Fang Ye^2AG*

DOI: 10.12659/MSM.929438

Med Sci Monit 2021; 27:e929438

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Table 1 Primers for quantitative polymerase chain reaction.

Gene sequences (5′ to 3′)
Collagen I	Forward	TAAGGGTCCCCAATGGTGAGA
Collagen I	Reverse	GGGTCCCTCGACTCCTACAT
Collagen III	Forward	TTGGAGAATCTATGAATGG
Collagen III	Reverse	GCAGTGGTATGTAATGTGTT
α-SMA	Forward	CCCAGACATCAGGGAGTAATGG
α-SMA	Reverse	TCTATCGGATACTTCAGCGTCA
TLR4	Forward	TTGTATCGCCTTCTTAGC
TLR4	Reverse	CTTCTTGTTCTTCCTCTGA
MyD88	Forward	TCATGTTCTCCATACCCTTGGT
MyD88	Reverse	AAACTGCGAGTGGGGTCAG
TRAF6	Forward	CATCTTCAGTTACCGACAGCTCAG
TRAF6	Reverse	TGGTCGAGAATTGTAAGGCGTAT

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Zi Qi Decoction Alleviates Liver Fibrosis by Inhibiting the Toll-Like Receptor 4 (TLR4)-Related Nuclear Factor kappa b (NF-κB) and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways

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