14 April 2021 : Animal Research
Zi Qi Decoction Alleviates Liver Fibrosis by Inhibiting the Toll-Like Receptor 4 (TLR4)-Related Nuclear Factor kappa b (NF-κB) and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways
Jingwen Zhou1ABCE, Xiaolong Zhang1A, Lingfeng Wan1AD, Jun Yu2BCD, Tianci Li2BCD, Ziyu Lu3BCD, Nanyuan Fang1E, Lixia Sun1AG, Fang Ye2AG*DOI: 10.12659/MSM.929438
Med Sci Monit 2021; 27:e929438
Table 1 Primers for quantitative polymerase chain reaction.
Gene sequences (5′ to 3′) | ||
---|---|---|
Collagen I | Forward | TAAGGGTCCCCAATGGTGAGA |
Reverse | GGGTCCCTCGACTCCTACAT | |
Collagen III | Forward | TTGGAGAATCTATGAATGG |
Reverse | GCAGTGGTATGTAATGTGTT | |
α-SMA | Forward | CCCAGACATCAGGGAGTAATGG |
Reverse | TCTATCGGATACTTCAGCGTCA | |
TLR4 | Forward | TTGTATCGCCTTCTTAGC |
Reverse | CTTCTTGTTCTTCCTCTGA | |
MyD88 | Forward | TCATGTTCTCCATACCCTTGGT |
Reverse | AAACTGCGAGTGGGGTCAG | |
TRAF6 | Forward | CATCTTCAGTTACCGACAGCTCAG |
Reverse | TGGTCGAGAATTGTAAGGCGTAT |