08 December 2021: Lab/In Vitro Research
Chemokine (C-C Motif) Ligand 2/Chemokine Receptor 2 (CCR2) Axis Blockade to Delay Chondrocyte Hypertrophy as a Therapeutic Strategy for Osteoarthritis
Zidong Wang 1ACE , Bei Wang 2CF , Jian Zhang 1DF , Zhensong Wu 3BD , Liankui Yu 1BF , Zhongye Sun 1ADG*DOI: 10.12659/MSM.930053
Med Sci Monit 2021; 27:e930053
Table 1 Primer sequences for RT-PCR.
| Gene name | Forward (5′>3′) | Reverse (5′>3′) |
|---|---|---|
| Collagen X | ATGCTGCCACAAATACCCTTT | GGTAGTGGGCCTTTTATGCCT |
| Collagen II | TGGACGATCAGGCGAAACC | GCTGCGGATGCTCTCAATCT |
| Runx-2 | TGGTTACTGTCATGGCGGGTA | TCTCAGATCGTTGAACCTTGCTA |
| Ihh | GGACGCTATGAAGGCAAGATCG | CAGCGAGTTCAGGCGGTCCTT |
| CCL2 | AGAATCACCAGCAGCAAGTGTCC | TCCTGAACCCACTTCTGCTTGG |
| CCR2 | CAGGTGACAGAGACTCTTGGGA | GGCAATCCTACAGCCAAGAGCT |
| GAPDH | ACAACTTTGGTATCGTGGAAGG | GCCATCACGCCACAGTTTC |