Knockout of the Cannabinoid Receptor 2 Gene Promotes Inflammation and Hepatic Stellate Cell Activation by Promoting A20/Nuclear Factor-κB (NF-κB) Expression in Mice with Carbon Tetrachloride-Induced Liver Fibrosis

Cuizhen Long; Na Xie; Yuanhui Shu; Yafeng Wu; Ping He; Yan Zhou; Yining Xiang; Junying Gu; Lei Yang; Yuping Wang

doi:10.12659/MSM.931236

20 August 2021 : Animal Research

Knockout of the Cannabinoid Receptor 2 Gene Promotes Inflammation and Hepatic Stellate Cell Activation by Promoting A20/Nuclear Factor-κB (NF-κB) Expression in Mice with Carbon Tetrachloride-Induced Liver Fibrosis

Cuizhen Long^123ABCDEF, Na Xie^12ABCDEF, Yuanhui Shu^12ABCF, Yafeng Wu^14ABCF, Ping He^12BC, Yan Zhou^12BC, Yining Xiang^5CD, Junying Gu^12CD, Lei Yang^12DF, Yuping Wang^12ABCDEFG*

DOI: 10.12659/MSM.931236

Med Sci Monit 2021; 27:e931236

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Table 2 Primer sequences used for reverse transcription-quantitative PCR.

Gene name	Primer sequences (5′-3′)
Gene name	Forward	Reverse
GAPDH	AAGAAGGTGGTGAAGCAGGCATC	CGGCATCGAAGGTGGAAGAGTG
TNF-α	CGACGTGGAACTGGCAGAAG	CACAAGCAGGAATGAGAAGAGG
IL-6	ACTTCCATCCAGTTGCCTTCTTGG	CCTCCGACTTGTGAAGTGG

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Knockout of the Cannabinoid Receptor 2 Gene Promotes Inflammation and Hepatic Stellate Cell Activation by Promoting A20/Nuclear Factor-κB (NF-κB) Expression in Mice with Carbon Tetrachloride-Induced Liver Fibrosis

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