20 August 2021>: Animal Study
Knockout of the Cannabinoid Receptor 2 Gene Promotes Inflammation and Hepatic Stellate Cell Activation by Promoting A20/Nuclear Factor-κB (NF-κB) Expression in Mice with Carbon Tetrachloride-Induced Liver Fibrosis
Cuizhen Long 123ABCDEF* , Na Xie 12ABCDEF* , Yuanhui Shu 12ABCF , Yafeng Wu 14ABCF , Ping He 12BC , Yan Zhou 12BC , Yining Xiang 5CD , Junying Gu 12CD , Lei Yang 12DF , Yuping Wang 12ABCDEFG*DOI: 10.12659/MSM.931236
Med Sci Monit 2021; 27:e931236
Table 2 Primer sequences used for reverse transcription-quantitative PCR.
Gene name | Primer sequences (5′-3′) | |
---|---|---|
Forward | Reverse | |
GAPDH | AAGAAGGTGGTGAAGCAGGCATC | CGGCATCGAAGGTGGAAGAGTG |
TNF-α | CGACGTGGAACTGGCAGAAG | CACAAGCAGGAATGAGAAGAGG |
IL-6 | ACTTCCATCCAGTTGCCTTCTTGG | CCTCCGACTTGTGAAGTGG |