04 June 2021: Animal Study
Effects of β-Asarone on Ischemic Stroke in Middle Cerebral Artery Occlusion Rats by an Nrf2-Antioxidant Response Elements (ARE) Pathway-Dependent Mechanism
Huiying Pan 1BC , Yi Xu 2D , Qian Cai 1E , Meiling Wu 1ABD , Mingxing Ding 1AG*DOI: 10.12659/MSM.931884
Med Sci Monit 2021; 27:e931884
Table 1 The primer sequence information for RT-qPCR.
| Gene name | 5′-3′ sequence | Product size | |
|---|---|---|---|
| Rat GAPDH | Forward | ACTCCCATTCTTCCACCTTTG | 105 bp |
| Reverse | CCCTGTTGCTGTAGCCATATT | ||
| Rat Nrf2 | Forward | CCCATTGAGGGCTGTGAT | 247 bp |
| Reverse | TTGGCTGTGCTTTAGGTC | ||
| Rat NQO1 | Forward | GAACTTTAACCCCCTCATTTCC | 237 bp |
| Reverse | CCTGCTACAAGCACTCTCTCAA | ||
| Rat GCLM | Forward | CCACCAGATTTGACTGCATTT | 178 bp |
| Reverse | TTTTCACGATGACCGAGTACC | ||
| Rat HO-1 | Forward | GCATGTCCCAGGATTTGTCC | 192 bp |
| Reverse | GGTTCTGCTTGTTTCGCTCT | ||
| Rat GCLC | Forward | ACAGTGGAGGACAACATGAGG | 232 bp |
| Reverse | GATGCCGGATGTTTCTTGTTA |