10 May 2022 : Laboratory Research
Impact of Factors Secreted by Tumor Cells on Response of Pleural Mesothelial Cells to Different Sclerosing Agents in an In Vitro Model
Michał Mierzejewski
1ABEF, Magdalena Paplińska-Goryca 1ABCDE*, Rafał Krenke 1ADEG
DOI: 10.12659/MSM.936065
Med Sci Monit 2022; 28:e936065
Table 1 Sequence of primers used in PCR.
| Forward primer | Reverse primer | Product size | |
|---|---|---|---|
| 18s rRNA | GGATGAGGTGGAACGTGTGAT | AGGTCTTCACGGAGCTTGTTG | 148 |
| IL-6 | CCGGGAACGAAAGAGAAGCT | GCGCTTGTGGAGAAGGAGTT | 67 |
| IL-8 | GAGCACACAAGCTTCT | ATCAGGAAGGCTGCCAAGAG | 114 |
| MMP9 | GCTCACCTTCACTCGCGTG | CGCGACACCAAACTGGATG | 61 |
| MCP-1 | GAGAGGCTGAGACT | GTGCGAGCTTCAGTTTGAGAATT | 52 |
| TGF-β | CAGCAACAATTCCTGGCGATA | AAGGCGAAAGCCCTCAATTT | 136 |
| IL-17A | AGGAATCACAATCCCACGAAAT | GGTGAGGTGGATCGGTTGTAGT | 149 |
| IL-1β | qHsaCIP0033362 | 119 | |