08 August 2022: Clinical Research
Identification of Hepatic Dendritic Cells in Liver Biopsies in Patients with Metabolic Dysfunction-Associated Fatty Liver Diseas (MAFLD) and Obesity
Beatriz Barranco-Fragoso 12ABC , Shreya C. Pal 34CDEFG , Luis E. Díaz-Orozco 34ABCDEF , Rita Dorantes-Heredia 5BCD , Xingshun Qi 6F , Nahum Méndez-Sánchez 34ACDEF*DOI: 10.12659/MSM.937528
Med Sci Monit 2022; 28:e937528
Table 1 Operational definitions for histopathological diagnosis of liver injury:The histological diagnoses established by the expert pathologist are presented below. These are written-out here for reference to the terms used within the text.
| Histological diagnoses | Operational definitions |
|---|---|
| Ballooning degeneration | Hepatocytes greater in size compared to adjacent hepatocytes, with rounded shape (instead of regular polygonal shape), vacuolization, and cytoplasmic clearing. Finding of Mallory hyaline bodies supports this diagnosis |
| Simple steatosis | In this case, the expert pathologist considered the definition of “macrovesicular steatosis” rather than “microvesicular steatosis” given that it’s the most common form seen overall and is the variant present in steatohepatitis, obesity, and diabetes, among others.Macrovesicular steatosis: “Large” fat droplets that are greater than 20 μm, cells with peripherally pushed nucleus, usually a single lipid droplet inside the cell |
| Inflammation | Presence of leukocyte infiltrate within tissues, consisting of neutrophils and macrophages in acute inflammatory processes and lymphocytes in chronic stages |
| Fibrosis | Collagen deposition which may or may not have architectural distortion within hepatic tissue; collagen fibers are seen as blue colored when evaluated with Masson stain. The degree of fibrosis was classified based on the Kleiner classification for liver steatosis |