09 September 2023 : Clinical Research
Synergistic Role of Biofilm-Associated Genes and Efflux Pump Genes in Tigecycline Resistance of Acinetobacter baumannii
Bin Luo1AE*, Zhiwei Li1B, Qian Wang2C, Changmin Wang2FDOI: 10.12659/MSM.940704
Med Sci Monit 2023; 29:e940704
Table 1 Target gene PCR primer sequences and product lengths.
| Genes | 5′-3′ | Product length |
|---|---|---|
| blaOXA-23-F | GATCGGATTGGAGAACCAGA | 501 |
| blaOXA-23-R | ATT TCTGACCGCATT TCCAT | |
| blaOXA-51-F | TAATGCTTTGATCGGCCTTG | 353 |
| blaOXA-51-R | TGGATTGCACTTCATCTTGG | |
| blaOXA-58-F | AAGTATTGGGGCTTGTGCTG | 599 |
| blaOXA-58-R | CCCCTCTGCGCTCTACATAC | |
| adeB (16)-F | ATTCGTCGTCCTGTTTTTGC | 271 |
| adeB (126)-R | CGGAGCTACACTTGGAAAGC | |
| adeA (118)-F | ATTCAACCGCAATCGGTAAA | 280 |
| adeA (260)-R | GCTTGCCCTGCTCTAACTTC | |
| AdeC 2F(AN) | TGTCGAAATCGGCAACTGTA | 310 |
| AdeC 159R(AN) | TTGTTCAGCAATCGTTTTTCC | |
| adeRS_1F(an) | ATGTTCGATCATTCTTTTTCTTTTG | 272 |
| adeRS_1849R(an) | TTACTAATCCATAGAAATTTTTATG | |
| abaI-F | GTACAGTCGACGTATTTGTTGAATATTTGGG | 382 |
| abaI-R | CGTACGTCTAGAGTAATGAGTTGTTTTGCGCC | |
| bap-F | CGTTTCCTGGGTCTGATGTATT | 942 |
| bap-R | GTTATTGAAGGCTTCTTTAGTG | |
| bfmS-F | CGTATGCATCAGGTCGAC | 355 |
| bfmS-R | ACAGACAAAAGCCTGCC | |
| bfmR-F | CACCATGAGCCAAGAAGAAAAG | 750 |
| bfmR-R | GACCAACCTTATAGGAAG | |
| bfs-F | GCGCATATGAAAAATGATGCAAATATC | 490 |
| bfs-R | GCGCTCGAGTCATTTCAAATCATATCGAG | |
| csuAB-F | ATGAATATGAAAAACATTCA | 530 |
| csuAB-R | TTAGAAATTTACAGTGACTA | |
| csuE-F | CATCTTCTATTTCGGTCCC | 372 |
| csuE-R | CGGTCTGAGCATTGGTAA | |
| DNA-N-F | TTTGGCTTCCTCTACTTC | 500 |
| DNA-N-R | CATCTTGTTCAGGGTTAT |