06 August 2023>: Article
Dynamics of Microbiome Changes in the Endometrium and Uterine Cervix during Embryo Implantation: A Comparative Analysis
Anna Bednarska-Czerwińska 123ABCDEF* , Emilia Morawiec 14ABDF , Nikola Zmarzły 3CDEF , Michał Szapski 1B , Justyna Jendrysek 1B , Anika Pecyna 1B , Karolina Zapletał-Pudełko 1B , Weronika Małysiak 1B , Tomasz Sirek 56E , Piotr Ossowski 3F , Aleksandra Łach 3E , Dariusz Boroń 3789D , Paweł Bogdał 3F , Adam Bernet 9F , Damian Strojny 3F , Beniamin Oskar Grabarek 13789AEDOI: 10.12659/MSM.941289
Med Sci Monit 2023; 29:e941289
Table 3 Bacterial microbiome composition of the cervix and endometrium.
Microbiome | 1st collection N (%) | 2nd collection N (%) | 3rd collection N (%) | p* | 1st collection Me (Q1–Q3) | 2nd collection Me (Q1–Q3) | 3rd collection Me (Q1–Q3) | p** |
---|---|---|---|---|---|---|---|---|
1 (0.6) | 0 (0) | 0 (0) | – | 1.4 (1.4–1.4) | 0 (0–0) | 0 (0–0) | – | |
0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1.3 (1.3–1.3) | 0 (0–0) | – | |
0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 13.3 (13.3–13.3) | 0 (0–0) | – | |
0 (0) | 0 (0) | 1 (0.6) | – | 0 (0–0) | 0 (0–0) | 1.2 (1.2–1.2) | – | |
3 (1.7) | 1 (0.6) | 0 (0) | – | 10 (2.5–18.4) | 2.1 (2.1–2.1) | 0 (0–0) | – | |
group | 2 (1.1) | 2 (1.1) | 0 (0) | – | 3.1 (1.9–4.3) | 2.1 (1.8–2.4) | 0 (0–0) | – |
group | 1 (0.6) | 0 (0) | 0 (0) | – | 1.8 (1.8–1.8) | 0 (0–0) | 0 (0–0) | – |
0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 3.1 (3.1–3.1) | 0 (0–0) | – | |
0 (0) | 1 (0.6) | 2 (1.1) | – | 0 (0–0) | 91.8 (91.8–91.8) | 32.6 (7.1–58.2) | – | |
0 (0) | 2 (1.1) | 1 (0.6) | – | 0 (0–0) | 2.7 (1.6–3.9) | 1.6 (1.6–1.6) | – | |
group | 0 (0) | 0 (0) | 1 (0.6) | – | 0 (0–0) | 0 (0–0) | 3.2 (3.2–3.2) | – |
0 (0) | 0 (0) | 1 (0.6) | – | 0 (0–0) | 0 (0–0) | 3.2 (3.2–3.2) | – | |
group | 0 (0) | 1 (0.6) | 1 (0.6) | – | 0 (0–0) | 10.3 (10.3–10.3) | 3.9 (3.9–3.9) | – |
sp. | 0 (0) | 1 (0.6) | 3 (1.7) | – | 0 (0–0) | 3.5 (3.5–3.5) | 1.6 (1–2.8) | – |
0 (0) | 0 (0) | 2 (1.1) | – | 0 (0–0) | 0 (0–0) | 2 (1.6–2.4) | – | |
spp. | 0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 2 (2–2) | 0 (0–0) | – |
spp. | 1 (0.6) | 0 (0) | 0 (0) | – | 1 (1–1) | 0 (0–0) | 0 (0–0) | – |
2 (1.1) | 2 (1.1) | 0 (0) | – | 50.9 (15.4–86.4) | 10.4 (5.9–14.8) | 0 (0–0) | – | |
1 (0.6) | 1 (0.6) | 1 (0.6) | – | 1.5 (1.5–1.5) | 3.3 (3.3–3.3) | 6.3 (6.3–6.3) | – | |
4 (2.3) | 10 (5.6) | 5 (2.8) | 0.18 | 39.8 (14.3–65.2) | 17.5 (4.6–17.7) | 38. (30.2–52.5) | – | |
0 (0) | 0 (0) | 1 (0.6) | – | 0 (0–0) | 0 (0–0) | 22.9 (22.9–22.9) | – | |
5 (2.8) | 6 (3.4) | 6 (3.4) | 0.94 | 17 (7.7–28.2) | 29.5 (2.3–66.5) | 16.6 (2.6–12) | 0.37 | |
0 (0) | 0 (0) | 1 (0.6) | – | 0 (0–0) | 0 (0–0) | 1.5 (1.5–1.5) | – | |
spp. | 0 (0) | 2 (1.1) | 0 (0) | – | 0 (0–0) | 12 (1.8–22.1) | 0 (0–0) | – |
1 (0.6) | 0 (0) | 0 (0) | – | 2.4 (2.4–2.4) | 0 (0–0) | 0 (0–0) | – | |
sp. | 1 (0.6) | 0 (0) | 1 (0.6) | – | 1.2 (1.2–1.2) | 0 (0–0) | 3.5 (3.5–3.5) | – |
1 (0.6) | 0 (0) | 0 (0) | – | 2.1 (2.1–2.1) | 0 (0–0) | 0 (0–0) | – | |
group | 0 (0) | 0 (0) | 1 (0.6) | – | 0 (0–0) | 0 (0–0) | 4.3 (4.3–4.3) | – |
group | 0 (0) | 0 (0) | 1 (0.6) | – | 0 (0–0) | 0 (0–0) | 1.3 (1.3–1.3) | – |
sp. | 0 (0) | 1 (0.6) | 3 (1.7) | – | 0 (0–0) | 18 (18–18) | 1.2 (1.1–1.4) | – |
group | 2 (1.1) | 1 (0.6) | 0 (0) | – | 3.9 (1–6.8) | 4.1 (4.1–4.1) | 0 (0–0) | – |
group | 0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1.6 (1.6–1.6) | 0 (0–0) | – |
group | 0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1.5 (1.5–1.5) | 0 (0–0) | – |
group | 1 (0.6) | 0 (0) | 0 (0) | – | 1.7 (1.7–1.7) | 0 (0–0) | 0 (0–0) | – |
0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1 (1–1) | 0 (0–0) | – | |
KQ235774s | 1 (0.6) | 0 (0) | 0 (0) | – | 18.2 (18.2–18.2) | 0 (0–0) | 0 (0–0) | – |
spp. | 1 (0.6) | 1 (0.6) | 0 (0) | – | 22.7 (22.7–22.7) | 8.5 (8.5–8.5) | 0 (0–0) | – |
group | 1 (0.6) | 1 (0.6) | 2 (1.1) | – | 31.3 (31.3–31.3) | 95.4 (95.4–95.4) | 42.2 (11.3–73) | – |
4 (2.3) | 10 (5.6) | 5 (2.8) | 0.18 | 11.4 (2.5–20.3) | 21.2 (2.6–24.2) | 6.1 (3.4–5.3) | – | |
group | 4 (2.3) | 15 (8.5) | 13 (7.3) | 0.03 | 25.2 (1.4–49) | 38.6 (2.9–72.9) | 52.8 (26.9–86.2) | – |
0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1.8 (1.8–1.8) | 0 (0–0) | – | |
2 (1.1) | 4 (2.3) | 5 (2.8) | – | 3.4 (2.4–4.4) | 1.9 (1.7–2.2) | 6.3 (2–8.7) | – | |
1 (0.6) | 0 (0) | 0 (0) | – | 1.5 (1.5–1.5) | 0 (0–0) | 0 (0–0) | – | |
group | 0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 2.8 (2.8–2.8) | 0 (0–0) | – |
group | 12 (6.8) | 10 (5.6) | 11 (6.2) | 0.91 | 19.6 (8–30.4) | 34.4 22.4–40.9) | 27.4 (2.8–38.9) | 0.25 |
group | 2 (1.1) | 0 (0) | 1 (0.6) | – | 3.3 (3.2–3.3) | 0 (0–0) | 9 (9–9) | – |
1 (0.6) | 1 (0.6) | 0 (0) | – | 3.1 (3.1–3.1) | 5.1 (5.1–5.1) | 0 (0–0) | – | |
group | 0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 7.4 (7.4–7.4) | 0 (0–0) | – |
0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 16.4 (16.4–16.4) | 0 (0–0) | – | |
spp. | 1 (0.6) | 0 (0) | 1 (0.6) | – | 1.2 (1.2–1.2) | 0 (0–0) | 9.1 (9.1–9.1) | – |
sp. | 1 (0.6) | 0 (0) | 0 (0) | – | 2.1 (2.1–2.1) | 0 (0–0) | 0 (0–0) | – |
1 (0.6) | 0 (0) | 0 (0) | – | 2.1 (2.1–2.1) | 0 (0–0) | 0 (0–0) | – | |
4 (2.3) | 4 (2.3) | 4 (2.3) | – | 3.6 (1.3–6) | 3 (1.5–4.4) | 2 (1.6–2.3) | – | |
1 (0.6) | 0 (0) | 0 (0) | – | 75.4 (75.4–75.4) | 0 (0–0) | 0 (0–0) | – | |
group | 1 (0.6) | 1 (0.6) | 1 (0.6) | – | 20.9 (20.9–20.9) | 1.2 (1.2–1.2) | 5.3 (5.3–5.3) | – |
6 (3.4) | 5 (2.8) | 2 (1.1) | – | 6.9 (2–4.8) | 4.4 (1.2–6.4) | 18.8 (7.3–30.2) | – | |
FN667084s | 0 (0) | 0 (0) | 2 (1.1) | – | 0 (0–0) | 0 (0–0) | 1.3 (1–1.6) | – |
group | 41 (23.2) | 35 (19.8) | 37 (20.9) | 0.73 | 60.9 (26.6–96.3) | 40.8 (4.1–81.1) | 42.7 (9.3–68.8) | 0.35 |
group | 125 (70.6) | 116 (65.5) | 118 (66.7) | 0.56 | 82.8 (87.9–99.7) | 83.7 (80.6–99.5) | 86.7 (83.7–99.6) | 0.03 |
23 (13) | 25 (14.1) | 25 (14.1) | 0.94 | 48 (13.1–87.8) | 56.8 (15.1–98.2) | 67 (33.8–98.3) | 0.19 | |
2 (1.1) | 1 (0.6) | 1 (0.6) | – | 0.9 (0.7–1.2) | 7.1 (7.1–7.1) | 4.2 (4.2–4.2) | – | |
group | 10 (5.6) | 7 (4) | 5 (2.8) | 0.41 | 9.9 (2.1–8.7) | 22.7 (4.8–24.3) | 9.4 (2.8–19.8) | – |
group | 1 (0.6) | 3 (1.7) | 4 (2.3) | – | 2.3 (2.3–2.3) | 2 (1–3.9) | 2.3 (1.5–3.2) | – |
group | 16 (9) | 12 (6.8) | 12 (6.8) | 0.65 | 4.4 (1.6–4.8) | 2.5 (1.5–3.6) | 3.5 (1.3–4.2) | 0.72 |
sp. | 3 (1.7) | 2 (1.1) | 4 (2.3) | – | 2.1 (1.9–2.2) | 1.8 (1.4–2.2) | 2.4 (1.7–3.2) | – |
group | 53 (29.9) | 45 (25.4) | 43 (24.3) | 0.44 | 35.5 (4.1–69.9) | 38.3 (7.8–71.9) | 32.7 (6–63.1) | 0.23 |
1 (0.6) | 0 (0) | 1 (0.6) | – | 1.5 (1.5–1.5) | 0 (0–0) | 2.3 (2.3–2.3) | – | |
0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1.2 (1.2–1.2) | 0 (0–0) | – | |
spp. | 1 (0.6) | 0 (0) | 0 (0) | – | 9.3 (9.3–9.3) | 0 (0–0) | 0 (0–0) | – |
group | 0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1.5 (1.5–1.5) | 0 (0–0) | – |
group | 1 (0.6) | 0 (0) | 0 (0) | – | 1.9 (1.9–1.9) | 0 (0–0) | 0 (0–0) | – |
group | 1 (0.6) | 0 (0) | 0 (0) | – | 21.4 (21.4–21.4) | 0 (0–0) | 0 (0–0) | – |
0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1 (1–1) | 0 (0–0) | – | |
group | 1 (0.6) | 0 (0) | 0 (0) | – | 4 (4–4) | 0 (0–0) | 0 (0–0) | – |
group | 1 (0.6) | 0 (0) | 0 (0) | – | 1.7 (1.7–1.7) | 0 (0–0) | 0 (0–0) | – |
spp. | 1 (0.6) | 1 (0.6) | 0 (0) | – | 11.5 (11.5–11.5) | 43.4 (43.4–43.4) | 0 (0–0) | – |
0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1.5 (1.5–1.5) | 0 (0–0) | – | |
group | 3 (1.7) | 1 (0.6) | 4 (2.3) | – | 2.3 (2–2.5) | 1.1 (1.1–1.1) | 3.4 (1.6–5.3) | – |
0 (0) | 0 (0) | 1 (0.6) | – | 0 (0–0) | 0 (0–0) | 1.1 (1.1–1.1) | – | |
1 (0.6) | 0 (0) | 3 (1.7) | – | 7.1 (7.1–7.1) | 0 (0–0) | 10.4 (2–26.4) | – | |
0 (0) | 0 (0) | 1 (0.6) | – | 0 (0–0) | 0 (0–0) | 2.1 (2.1–2.1) | – | |
group | 1 (0.6) | 0 (0) | 0 (0) | – | 1.6 (1.6–1.6) | 0 (0–0) | 0 (0–0) | – |
sp. | 1 (0.6) | 0 (0) | 0 (0) | – | 1.3 (1.3–1.3) | 0 (0–0) | 0 (0–0) | – |
group | 1 (0.6) | 0 (0) | 0 (0) | – | 1.4 (1.4–1.4) | 0 (0–0) | 0 (0–0) | – |
group | 0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 2.8 (2.8–2.8) | 0 (0–0) | – |
0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1.4 (1.4–1.4) | 0 (0–0) | – | |
0 (0) | 0 (0) | 0 (0) | – | 0 (0–0) | 0 (0–0) | 0 (0–0) | – | |
sp. | 1 (0.6) | 1 (0.6) | 0 (0) | – | 3.4 (3.4–3.4) | 8 (8–8) | 0 (0–0) | – |
0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1.1 (1.1–1.1) | 0 (0–0) | – | |
1 (0.6) | 0 (0) | 0 (0) | – | 1.8 (1.8–1.8) | 0 (0–0) | 0 (0–0) | – | |
sp. | 1 (0.6) | 0 (0) | 0 (0) | – | 1.7 (1.7–1.7) | 0 (0–0) | 0 (0–0) | – |
group | 8 (4.5) | 20 (11.3) | 14 (7.9) | 0.06 | 9.8 (1.5–14.2) | 14.1 (2.7–15.9) | 8.6 (1.6–10.5) | – |
group | 0 (0) | 0 (0) | 1 (0.6) | – | 0 (0–0) | 0 (0–0) | 2.2 (2.2–2.2) | – |
0 (0) | 1 (0.6) | 1 (0.6) | – | 0 (0–0) | 63.4 (63.4–63.4) | 26.3 (26.3–26.3) | – | |
group | 0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1.1 (1.1–1.1) | 0 (0–0) | – |
5 (2.8) | 5 (2.8) | 7 (4) | 0.78 | 27.1 (7.7–25.6) | 20.8 (4.6–20) | 21.7 (3.2–47.3) | – | |
group | 3 (1.7) | 6 (3.4) | 7 (4) | – | 19.6 (2.4–51.8) | 3.1 (2.3–4.1) | 14.5 (1.3–11.6) | – |
group | 0 (0) | 6 (3.4) | 6 (3.4) | – | 0 (0–0) | 41.9 (1.1–95.2) | 18.6 (1.5–19.6) | – |
group | 2 (1.1) | 8 (4.5) | 2 (1.1) | – | 7.8 (7.8–7.8) | 19.1 (2.9–20.2) | 1.5 (1.5–1.5) | – |
group | 2 (1.1) | 4 (2.3) | 7 (4) | – | 14.7 (6.5–22.8) | 15.2 (2.8–27.5) | 29.2 (1.9–39.6) | – |
group | 2 (1.1) | 2 (1.1) | 0 (0) | – | 9.7 (8.6–10.8) | 2.7 (1.7–3.7) | 0 (0–0) | – |
1 (0.6) | 1 (0.6) | 3 (1.7) | – | 2.5 (2.5–2.5) | 10.7 (10.7–10.7) | 9.4 (1.1–18.2) | – | |
0 (0) | 0 (0) | 1 (0.6) | – | 0 (0–0) | 0 (0–0) | 1.2 (1.2–1.2) | – | |
0 (0) | 1 (0.6) | 0 (0) | – | 0 (0–0) | 1.2 (1.2–1.2) | 0 (0–0) | – | |
sp. | 0 (0) | 0 (0) | 1 (0.6) | – | 0 (0–0) | 0 (0–0) | 1.7 (1.7–1.7) | – |
Me – median; Q1–Q3 – quartiles 1 and 3. * Significance test result of percentage differences; ** Friedman’s test result. |