01 January 2000
Accuracy of t-PA, u-PA, PAI-1 and PAI-2 estimation in human bile by ELISA kits.
Zdzisława Kondera-Anasz, Aleksander Michalski, Dariusz Gil, Bożena Gil, Jacek Starzewski, Zbigniew GonciarzMed Sci Monit 2000; 6(3): PI616-617 :: ID: 421299
Abstract
The aim of this study was to establish the accuracy of determination offibrinolytic proteins in human bile using commercial kits. MATERIAL AND METHOD: Gall bladder bile wasobtained from patient undergoing laparoscopic cholecystectomy. The concentrations of tissue plasminogenactivator (tPA), urokinase (uPA) and plasminogen activator inhibitor-1 (PAI-1) and inhibitor-2 (PAI-2)in bile were measured by enzyme linked immunoassay. Afterwards we conducted so called 'recovery test'.RESULTS: Recovery percents of t-PA, u-PA, PAI-1, PAI 2 were very high i.e. 91.94, 94.07, 93.95, 91.2.We concluded that human fibrinolytic proteins in bile could be detected very accurately using commercialkits.
Keywords: Bile, Cholecystectomy, Laparoscopic, Enzyme-Linked Immunosorbent Assay, Plasminogen Activator Inhibitor 1, Plasminogen Activator Inhibitor 2, Reagent Kits, Diagnostic, Reproducibility of Results, Tissue Plasminogen Activator, Urinary Plasminogen Activator
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
12 Mar 2024 : Clinical Research
Comparing Neuromuscular Blockade Measurement Between Upper Arm (TOF Cuff®) and Eyelid (TOF Scan®) Using Miv...Med Sci Monit In Press; DOI: 10.12659/MSM.943630
11 Mar 2024 : Clinical Research
Enhancement of Frozen-Thawed Human Sperm Quality with Zinc as a Cryoprotective AdditiveMed Sci Monit In Press; DOI: 10.12659/MSM.942946
12 Mar 2024 : Database Analysis
Risk Factors of Age-Related Macular Degeneration in a Population-Based Study: Results from SHIP-TREND-1 (St...Med Sci Monit In Press; DOI: 10.12659/MSM.943140
12 Mar 2024 : Clinical Research
Preoperative Blood Transfusion Requirements for Hemorrhoidal Severe Anemia: A Retrospective Study of 128 Pa...Med Sci Monit In Press; DOI: 10.12659/MSM.943126
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952