03 June 2004
Mu opiate receptor subtypes
Patrick CadetMed Sci Monit 2004; 10(6): MS28-32 :: ID: 11688
Abstract
The m opiate receptor gene (MOR) has at least 14 exons that can generate 15 different splice variants. Recently, two new human MOR splice variants (hMOR-1O and hMOR-1X) have been identified and characterized. The two variants containing human MOR exons 1, 2, and 3 and a fourth alternative exon O, or exon X, are expressed in human brain tissue, and are selective for μ opioid binding in transfected cells. It is unclear; however, what the biologic role of these two novel human splice variants is in vivo. The μ3 opiate receptor subtype found in various human tissues where it is coupled to constitutive nitric oxide synthase derived nitric oxide release is characterized by its opiate alkaloid selectivity and its insensitivity to opioid peptides. The μ3 clone exhibits 100% identity to the μ1 receptor subtype in the center and conserved region, but with a truncated 5’-end (position 503 of μ1 mRNA) (missing several hundred nucleotides). In addition, the 3’-end of the new clone contains the 3’-end of the μ1 receptor, followed by a new fragment of 263 bases, and then by a 202 bp fragment of the 3’-end of the μ1 gene untranslated region. When μ3 is expressed in a heterologous system, the protein produced from this cDNA exhibits all of the expected biochemical characteristics of the μ3 receptor. The isolation of this novel splice variant adds support to the presence of morphinergic signaling in animals.
Keywords: Alternative Splicing, Base Sequence, Molecular Sequence Data, Receptors, Opioid, mu - classification, Receptors, Opioid, mu - genetics, Receptors, Opioid, mu - metabolism, Alternative Splicing, Base Sequence, Molecular Sequence Data, Receptors, Opioid, mu - metabolism
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
11 Mar 2024 : Clinical Research
Enhancement of Frozen-Thawed Human Sperm Quality with Zinc as a Cryoprotective AdditiveMed Sci Monit In Press; DOI: 10.12659/MSM.942946
12 Mar 2024 : Database Analysis
Risk Factors of Age-Related Macular Degeneration in a Population-Based Study: Results from SHIP-TREND-1 (St...Med Sci Monit In Press; DOI: 10.12659/MSM.943140
12 Mar 2024 : Clinical Research
Preoperative Blood Transfusion Requirements for Hemorrhoidal Severe Anemia: A Retrospective Study of 128 Pa...Med Sci Monit In Press; DOI: 10.12659/MSM.943126
12 Mar 2024 : Clinical Research
Tissue Inhibitors of Metalloproteinase 1 (TIMP-1) and 3 (TIMP-3) as New Markers of Acute Kidney Injury Afte...Med Sci Monit In Press; DOI: 10.12659/MSM.943500
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952