01 June 2006
Two stage EDTA anti-calcification method for bioprosthetic heart valve materials.
Nehir Sucu, Kerem Karaca, Nejat Yilmaz, Nejat Yilmaz, Barlas Aytacoglu, Lulufer Tamer, Murat Ozeren, H. Ali Dondas, Yzzet Oguz, Oya Ogenler, Murat DikmengilMed Sci Monit 2006; 12(6): MT33-38 :: ID: 451275
Abstract
Background: The aim of this study was to investigate the effect of two-stageEDTA treatment in diminishing calcific degeneration in bovine pericardial bioprosthetic heart valve material.Material/Methods: Conventionally preserved pericardium specimens were divided into two groups. GroupI (controls, n=18) pieces were first fixed in phosphate-buffered solution (PBS)+0.6% glutaraldehyde at+4 degrees C for 24 hours, then stored in PBS+0.2% glutaraldehyde at room temperature for 6 days. GroupII (study group, n=18) pieces were treated with PBS containing 100 microg/ml ethylenediaminetetraaceticacid (EDTA) at +4 degrees C for 24 hours, then fixed in PBS+0.6% glutaraldehyde as was group I at +4degrees C for 24 hours. After a second exposure to PBS containing 100 microg/ml EDTA at room temperaturefor 24 hours, they were stored in PBS+0.2% glutaraldehyde at room temperature for 4 days. Pericardialpatches were inserted into the dorsal pouches of 18 juvenile male Wistar rats. After 7 weeks of implantation,all the pericardium pieces were harvested from sacrificed rats. The calcium content and biomechanicalproperties of the explanted tissues were evaluated and also examined histopathologically. Results: Thedifference in the calcium content of the control and study groups was statistically significant. Biomechanicaland histopathologic assessment also supported these findings. Conclusions: Application of two-stage EDTAwas found to be useful in the attenuation of calcification in bioprosthetic heart valve materials withmildly increased durability. As calcification was reduced by approximately 50%, it can be consideredfor use with other agents as an adjuvant treatment.
Keywords: Bioprosthesis, Calcinosis - prevention & control, Calcium - analysis, Cattle, Edetic Acid - pharmacology, Heart Valve Prosthesis, Heart Valve Prosthesis Implantation, Pericardium - ultrastructure, Tissue Preservation - methods
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
12 Mar 2024 : Clinical Research
Metabolomic Alterations in Methotrexate Treatment of Moderate-to-Severe PsoriasisMed Sci Monit In Press; DOI: 10.12659/MSM.943360
14 Mar 2024 : Clinical Research
Renal Dysfunction Increases Risk of Adverse Cardiovascular Events in 5-Year Follow-Up Study of Intermediate...Med Sci Monit In Press; DOI: 10.12659/MSM.943956
15 Mar 2024 : Clinical Research
Impact of One-Lung Ventilation on Oxygenation and Ventilation Time in Thoracoscopic Heart Surgery: A Compar...Med Sci Monit In Press; DOI: 10.12659/MSM.943089
14 Mar 2024 : Clinical Research
Differential DHA and EPA Levels in Women with Preterm and Term Births: A Tertiary Hospital Study in IndonesiaMed Sci Monit In Press; DOI: 10.12659/MSM.943895
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952