01 July 1996
Expression of the binding sites for anti-zona pellucida antibodies in the oviduct
Zuzana Jirsova, Jindrich MartinekMed Sci Monit 1996; 2(4): BR391-396 :: ID: 499900
Abstract
Antibodies against zona pellucida (ZP) have recently aroused interest for their contraceptive activity in vitro and in vivo. In our study we attempted to use anti-ZP antibodies for detection of binding sites for ZP antigens on the oviductal epithelium considering the fact that in the course of fertilization and early stages of embryonic development the ZP is denuded and comes into intimate contact with the lining of the oviduct. The binding sites for anti-rat ZP antibodies (ARZPAb) prepared by immunization of a BALB/c mouse were detected using an indirect immunoperoxidase technique on the ZP of oviductal oocytes and on the luminal surface of the oviductal epithelium of the isthmus under the light microscope. For the electron microscopic detection of binding sites for anti-rat ZP antibodies post-embbedding immunogold technique was used. The binding sites were expressed in the ZP and on the apical membrane of the oviductal epithelium as well. The TEC-02 IgM monoclonal antibody recognizing the TEC-2 epitope carried on the ZP of a mouse egg was used for detection of the binding sites in the mouse. The binding of TEC-02 antibody was confirmed in the ZP of ovarian oocytes. In the fimbrial and ampullar parts of the oviduct the binding sites were detected on individual epithelial cells, in the isthmus the expression of TEC-2 epitope was more frequent. No binding sites for anti-ZP antibodies were found in the uterine and vaginal epithelium. The findings of binding of ARZPAb detected on the oviductal epithelium of the rat and mouse support the hypothesis of the presence of membrane receptors for ZP antigens in the oviduct.
Keywords: oviduct, Zona Pellucida, Binding Sites, Antibodies, antigen determinants
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
16 Mar 2024 : Clinical Research
Diagnostic Efficiency of ACR-TIRADS Score for Differentiating Benign and Malignant Thyroid Nodules of Vario...Med Sci Monit In Press; DOI: 10.12659/MSM.943228
08 May 2024 : Clinical Research
Effect of Individualized PEEP Guided by Driving Pressure on Diaphragm Function in Patients Undergoing Lapar...Med Sci Monit In Press; DOI: 10.12659/MSM.944022
21 Mar 2024 : Clinical Research
Impact of Serum Vitamin D, B6, and B12 and Cognitive Functions on Quality of Life in Peri- and Postmenopaus...Med Sci Monit In Press; DOI: 10.12659/MSM.943249
17 Mar 2024 : Clinical Research
Protective Role of TRPC3 Gene Polymorphism (rs10518289) in Obstructive Sleep Apnea Hypopnea Syndrome Among ...Med Sci Monit In Press; DOI: 10.12659/MSM.942667
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952