01 May 1997
Interaction of Zinc-phthalocyanine--tetrasulphonate with different types of bacterial cells
Fruzsina Gabor, Gabriella Csik, Gyorgyi RontoMed Sci Monit 1997; 3(3): BR294-298 :: ID: 500772
Abstract
Gram-negative Escherichia coli B. and Gram-positive Streptococcus faecalis were incubated with a water-soluble, second generation photosensitizer - Zn(II) - phthalocyanine - tetrasulphonate (ZnPcTS). The dark toxicity of the drug was checked at its different concentrations. Growth curves of treated and untreated cultures were assessed by turbidimetric measurement and compared by two-samples t-test. The quantity of incorporated phthalocyanine was determined by its fluorescence intensity and then correlated to the protein content of bacteria. ZnPcTS showed significantly greater uptake by Gram-positive S. faecalis than by Gram-negative E.coli B. The dye appears to interact with the two types of bacteria according to different pathways as suggested by the differences in temperature-dependence of the binding process. The amount of photosensitizer associated with the cells was also determined for different numbers of washing steps. The weakly bound fraction can be removed by two washing steps for both bacteria.
Keywords: Zinc-phthalocyanine-tetrasulphonate, cellular uptake, dark toxicity, photosensitization, Gram-Positive Bacteria, Gram-Negative Bacteria
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
14 Mar 2024 : Clinical Research
Renal Dysfunction Increases Risk of Adverse Cardiovascular Events in 5-Year Follow-Up Study of Intermediate...Med Sci Monit In Press; DOI: 10.12659/MSM.943956
15 Mar 2024 : Clinical Research
Impact of One-Lung Ventilation on Oxygenation and Ventilation Time in Thoracoscopic Heart Surgery: A Compar...Med Sci Monit In Press; DOI: 10.12659/MSM.943089
14 Mar 2024 : Clinical Research
Differential DHA and EPA Levels in Women with Preterm and Term Births: A Tertiary Hospital Study in IndonesiaMed Sci Monit In Press; DOI: 10.12659/MSM.943895
15 Mar 2024 : Clinical Research
Evaluation of an Optimized Workflow for the Radiofrequency Catheter Ablation of Paroxysmal Atrial FibrillationMed Sci Monit In Press; DOI: 10.12659/MSM.943526
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952