01 January 1998
Different intracellular calcium pools used by ethanol, vasopressin and bradykinin in perfused livers
Zoltan Ruttner, Laszlo Ligeti, Leslie Reinlib, Alan Charles McLaughlinMed Sci Monit 1998; 4(1): BR9-14 :: ID: 502803
Abstract
The effect of ethanol, vasopressin and bradykinin on cytosolic Ca2+ concentration ([Ca2+]i) levels was studied upon in the perfused livers obtained from Wistar rats. [Ca2+]i was measured using a fluorescence Ca2+ Indo-1 probe. The ED50 was found to be 500 mM for ethanol and 10 nM for vasopressin. Ethanol had a long lasting effect in elevating [Ca2+]i levels in contrast to the transient effect of vasopressin. Vasopressin failed to increase [Ca2+]i levels when applied after ethanol and vice versa. Depletion of intracellular Ca2+ stores by ryanodine and bradykinin (10 nM) had no effect on [Ca2+]i levels. In contrast, ethanol (500 mM) and vasopressin (10 nM) caused an increase in the calcium concentration level [Ca2+]i by 49% and 44% respectively, as compared to the control group. Perfusion with calcium free solution following ryanodine treatment resulted in an ineffectiveness of all three calcium agonists. This data suggest that ethanol and vasopressin increase the Ca2+ flux through the cell membrane and that they seem to influence the same intracellular calcium pools.
Keywords: Fluorometry, Ryanodine, Inositol, Rat
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
12 Mar 2024 : Clinical Research
Metabolomic Alterations in Methotrexate Treatment of Moderate-to-Severe PsoriasisMed Sci Monit In Press; DOI: 10.12659/MSM.943360
14 Mar 2024 : Clinical Research
Renal Dysfunction Increases Risk of Adverse Cardiovascular Events in 5-Year Follow-Up Study of Intermediate...Med Sci Monit In Press; DOI: 10.12659/MSM.943956
15 Mar 2024 : Clinical Research
Impact of One-Lung Ventilation on Oxygenation and Ventilation Time in Thoracoscopic Heart Surgery: A Compar...Med Sci Monit In Press; DOI: 10.12659/MSM.943089
14 Mar 2024 : Clinical Research
Differential DHA and EPA Levels in Women with Preterm and Term Births: A Tertiary Hospital Study in IndonesiaMed Sci Monit In Press; DOI: 10.12659/MSM.943895
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952