Contractility of smooth muscles and ultrastructure of their microfilaments in restriction of physical activity
Marija Kusleikaite, Sigitas Stonkus, Saulius KusleikaMed Sci Monit 2001; 7(1): BR34-37 :: ID: 510765
Abstract
Background: Depending on the intensity of hypodynamics (as s factor of ischemic heart disease) the aim of our investigation was to determine the contractility of smooth muscles of thoracic aorta with and without endothelium by biological active substances (prostaglandin F2α and acetylcholine) and to compare with ultrastructural alterations of microfilaments.
Material/Methods: A hypodynamic stress of 48-days duration was provoked via permanent and periodically recurrent intervation (when the hypodynamic state was altered with physical activity) in Chinchilla rabbits by placing them in metal hutches (according to B. M. Fiodorov). The relaxing effect of endothelium - dependent vasodilatator agonist (acetylcholine in concentration 10-7 M, 10-6 M, 10-5 M, 10-4 M) on smooth muscles (treated with vasoconstrictor prostaglandin F2α) was detected by means of a mechanotron 6 MXIC in isometric regime. The contraction force was expressed in percentage. One hundred percent was the maximum contraction that developed after administration of 2×10-5 M prostaglandin F2α solution to the smooth muscles preparation. For ultrastructure studies of microfilaments semithin sections of smooth muscles were examined with electron microscope 'Philips - 300'.
Results: The research findings show that under the influence of acetylcholine the relaxing of endothelium of thoracic aorta smooth muscles (treated with prostaglandin F2α) is more expressed in periodically recurrent intervation.
Conclusions: Permanent hypodynamic stress of 48 days duration caused strongly expressed contractility of smooth muscles with is associated with ultrastructural damage of microfilaments (such as irregular formation, drop out in flakes, microfilaments becoming shorter and thicker).
Keywords: contractility, smooth muscles, Endothelium, ultrastructure of microfilaments
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
07 May 2024 : Clinical Research
Treatment of AVN-Induced Proximal Pole Scaphoid Nonunion Using a Fifth and Fourth Extensor Compartmental Ar...Med Sci Monit In Press; DOI: 10.12659/MSM.944553
16 Mar 2024 : Clinical Research
Diagnostic Efficiency of ACR-TIRADS Score for Differentiating Benign and Malignant Thyroid Nodules of Vario...Med Sci Monit In Press; DOI: 10.12659/MSM.943228
08 May 2024 : Clinical Research
Effect of Individualized PEEP Guided by Driving Pressure on Diaphragm Function in Patients Undergoing Lapar...Med Sci Monit In Press; DOI: 10.12659/MSM.944022
21 Mar 2024 : Clinical Research
Impact of Serum Vitamin D, B6, and B12 and Cognitive Functions on Quality of Life in Peri- and Postmenopaus...Med Sci Monit In Press; DOI: 10.12659/MSM.943249
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952