Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 December 2004

Direct immunofluorescence on uninvolved, lesional and perilesional skin in patients with Endemic Pemphigus Foliaceus (Fogo Selvagem)

José Roberto Pereira Pegas, Vitor Manoel Silva dos Reis

Med Sci Monit 2004; 10(12): CR657-661 :: ID: 13231


Background:Research on direct immunofluorescence in pemphigus foliaceus (“fogo selvagem”) has been focused mainly on the study of perilesional and lesional skin, while little attention has been given to uninvolved skin. We analyzed the frequency of IgA, IgM, IgG and its subclasses (IgG1, IgG2, IgG3 and IgG4) and C3 complement fraction deposition in intercellular spaces (ICS) and basal membrane zone (BMZ) in uninvolved, lesional and perilesional skin from 47 fogo selvagem patients.Material/Methods:For each patient biopsies were collected from lesional, perilesional and uninvolved skin, and then analyzed by direct immunofluorescence. The panel of antibodies consisted of IgA, IgM, IgG and its subclasses (IgG1, IgG2, IgG3 and IgG4) and C3 complement fraction.Results:The results showed a predominance of IgG and IgG4 deposit in all skin samples, followed by C3 complement fraction and IgG1 deposits. The positive response for IgG on uninvolved (91.48%), lesional (93.61%) and perilesional (97.87%) skin was similar to that found for IgG4 in the same samples: 95.74%, 95.74% and 97.87%, respectively. Regarding IgG1, the uninvolved skin showed lower results (14.89%) than the lesional (29.78%) and perilesional skin (29.78%). Concerning C3 complement fraction, the perilesional skin showed higher results (40.42%) than the uninvolved and lesional skin (34.04% for both).Conclusions:The results suggest the importance of uninvolved skin for direct immunofluorescence in the diagnostics of pemphigus foliaceus. Our results suggest that any cutaneous region can demonstrate pemphigus antibodies by direct immunofluorescence.

Keywords: Complement C3 - analysis, Immunoglobulins - analysis, Pemphigus - diagnosis, Complement C3 - analysis, Fluorescent Antibody Technique, Direct, Immunoglobulins - analysis, Pemphigus - immunology, Skin - pathology

Add Comment 0 Comments


01 April 2024 : Editorial  

Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug Resistance

Dinah V. Parums

DOI: 10.12659/MSM.944600

Med Sci Monit 2024; 30:e944600


In Press

04 Mar 2024 : Clinical Research  

Injury-Admission Time is an Independent Risk Factor for Deep Vein Thrombosis in Older Patients with Osteopo...

Med Sci Monit In Press; DOI: 10.12659/MSM.943587  


04 Mar 2024 : Clinical Research  

Evaluation of the Interrater Reliability of Sonographic Measurements of Muscle Thickness of 38 Piriformis M...

Med Sci Monit In Press; DOI: 10.12659/MSM.943720  


05 Apr 2024 : Clinical Research  

Comparative Analysis of Transoral Endoscopic Parathyroidectomy Vestibular Approach and Focused Open Surgery...

Med Sci Monit In Press; DOI: 10.12659/MSM.944128  

05 Mar 2024 : Clinical Research  

Muscular Function Recovery from General Anesthesia in 132 Patients Undergoing Surgery with Acceleromyograph...

Med Sci Monit In Press; DOI: 10.12659/MSM.942780  

Most Viewed Current Articles

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799


14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990


16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387


01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952


Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750