Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 September 2005

Stability of infectious recombinant adeno-associated viral vector in gene delivery.

Ruian Xu, Mehdi Rahimi, Hsu Ma, Peter Fung, Chunqi Chang, Sue Xu, Matthew During

Med Sci Monit 2005; 11(9): BR305-308 :: ID: 428440

Abstract

Background: The aim of this study is to provide a basis for the designof appropriate protocols for the shipping and storage of rAAV vectors for experimental laboratory studiesand clinical trials. Material/Methods: rAAV stocks were generated by standard methods and then subjectedto different environments. The transduction efficiency of viral vectors both in vitro and in vivo wasdetermined by luciferase activity and immunohistochemistry. Results: The virus stored at -80 degreesC remained completely stable and had high transduction efficiency. By contrast, the transduction efficiencyof all other groups on 293 cells decreased continuously over time. The transduction efficiency of the-20 degrees C group remained relatively high for the first 5 days, but dropped sharply between days 5and 7. The transduction efficiency for the 4 degrees C group dropped sharply on both days 1 and 7, andcontinued to decrease to 55% of maximum efficiency by the end of the first month. For both the room temperature(RT) and 37 degrees C groups, a sharp fall in efficiency was observed at day 1, and efficiency continuedto decline throughout the experimental period. Data from the in vivo study also revealed that rAAV vectorstored at -80 degrees C remained stable and retained its transduction efficiency. Conclusions: The virusstored at -80 degrees C remained completely stable and retained high transduction efficiency. The implicationsof these findings provide a basis for viral stock portioning and avoidance of freeze-thawing and storingat temperatures above -80 degrees C prior to clinical trials.

Keywords: Cell Line, Cryopreservation, Dependovirus - genetics, genetic therapy, Genetic Vectors, Luciferases - genetics, Rats, Wistar, Recombinant Proteins - genetics, Recombination, Genetic, Temperature, Transduction, Genetic

Add Comment 0 Comments

834 150

Editorial

01 June 2023 : Editorial  

Editorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic Preparedness

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.941209

Med Sci Monit 2023; 29:e941209

0:00

In Press

05 Jun 2023 : Clinical Research  

Comparison of Texture and Color Enhancement Imaging with White Light Imaging in 52 Patients with Short-Segm...

Med Sci Monit In Press; DOI: 10.12659/MSM.940249  

05 Jun 2023 : Laboratory Research  

Comparison of Composite Resin (Duo-Shade) Shade Guide with Vita Ceramic Shades Before and After Chemical an...

Med Sci Monit In Press; DOI: 10.12659/MSM.940949  

02 Jun 2023 : Database Analysis  

The COVID-19 Crisis and the Incidence of Alcohol-Related Deaths in Poland

Med Sci Monit In Press; DOI: 10.12659/MSM.940904  

01 Jun 2023 : Clinical Research  

Root Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam ...

Med Sci Monit In Press; DOI: 10.12659/MSM.940472  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

0:00

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750