01 September 2005
Med Sci Monit 2005; 11(9): BR305-308 :: ID: 428440
Background: The aim of this study is to provide a basis for the designof appropriate protocols for the shipping and storage of rAAV vectors for experimental laboratory studiesand clinical trials. Material/Methods: rAAV stocks were generated by standard methods and then subjectedto different environments. The transduction efficiency of viral vectors both in vitro and in vivo wasdetermined by luciferase activity and immunohistochemistry. Results: The virus stored at -80 degreesC remained completely stable and had high transduction efficiency. By contrast, the transduction efficiencyof all other groups on 293 cells decreased continuously over time. The transduction efficiency of the-20 degrees C group remained relatively high for the first 5 days, but dropped sharply between days 5and 7. The transduction efficiency for the 4 degrees C group dropped sharply on both days 1 and 7, andcontinued to decrease to 55% of maximum efficiency by the end of the first month. For both the room temperature(RT) and 37 degrees C groups, a sharp fall in efficiency was observed at day 1, and efficiency continuedto decline throughout the experimental period. Data from the in vivo study also revealed that rAAV vectorstored at -80 degrees C remained stable and retained its transduction efficiency. Conclusions: The virusstored at -80 degrees C remained completely stable and retained high transduction efficiency. The implicationsof these findings provide a basis for viral stock portioning and avoidance of freeze-thawing and storingat temperatures above -80 degrees C prior to clinical trials.
Keywords: Cell Line, Cryopreservation, Dependovirus - genetics, genetic therapy, Genetic Vectors, Luciferases - genetics, Rats, Wistar, Recombinant Proteins - genetics, Recombination, Genetic, Temperature, Transduction, Genetic
01 August 2022 : EditorialEditorial: Long-Term Effects of Symptomatic and Asymptomatic SARS-CoV-2 Infection in Children and the Changing Pathogenesis of Common Childhood Viruses Driven by the COVID-19 Pandemic
Med Sci Monit 2022; 28:e937927
08 Aug 2022 : Clinical ResearchA 3-Year Clinical Evaluation of Endodontically Treated Posterior Teeth Restored with Resin Nanoceramic Comp...
Med Sci Monit In Press; DOI: 10.12659/MSM.937331
08 Aug 2022 : Clinical ResearchHemoglobin/Red Blood Cell Distribution Width Ratio in Peripheral Blood Is Positively Associated with Progno...
Med Sci Monit In Press; DOI: 10.12659/MSM.937146
05 Aug 2022 : Clinical ResearchA Retrospective Study of 98 Elderly Patients with High-Risk Lateral Femoral Wall Intertrochanteric Hip Frac...
Med Sci Monit In Press; DOI: 10.12659/MSM.936923
Most Viewed Current Articles
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292