Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

21 December 2009

Barrier functionality and transport machineries of human ECV304 cells

Jaleh BararABCEF, Mark GumbletonDEF, Masoud AsadiCF, Yadollah OmidiABCDEFG

Med Sci Monit 2010; 16(1): BR52-60 :: ID: 878321

Abstract

Background
The lack of a brain-derived endothelial cell-based model has led researchers to exploit non-brain endothelial/epithelial cells as in vitro models for blood-brain barrier (BBB) investigations. Of these, the endothelial-like human ECV304 cell line was initially characterized as endothelial cells and has been widely used as an in vitro BBB model. It was later shown to be T24 bladder carcinoma epithelial cells. To pursue its potential as a cell-based model for drug screening and transport machineries, ECV304 cells were evaluated for their barrier and plasma membrane characteristics.
Material and Method
ECV304 cells treated with some tight-junction modulators (e.g. cAMP elevators, butyric acid, dexamethasone, gamma-linolenic acid, and astrocytic factors) were examined for bioelectrical resistance, transcellular/paracellular permeability, and functionality expression of some key transporters.
Results
A significant (p<0.05) increase in trans-endothelial electrical resistance (TEER) was observed in ECV304 cells treated with astrocytic factors and cAMP elevators (i.e. to approximately 110Omega*cm2 vs. approximately 60 Omega*cm2 in the control). Such treatment also yielded high discrimination in the permeability coefficients of the transcellular marker propranolol (25x10-6 cm/sec) and the paracellular marker sucrose (11.3 x 10-6 cm/sec). Carrier-mediated transporters such as GLUT-1, system L, and P-glycoprotein (P-gp) as well as endocytic transport machineries (e.g. clathrin and caveolin) were found to be functionally expressed.
Conclusions
ECV304 cells failed to generate a discriminative tight barrier even in the presence of tight-junction modulators. Thus their implementation in drug permeability screening is not recommended. They display some important key carrier-mediated transport systems and can be considered as a useful cell-based in vitro model.

Keywords: Models, Biological, Membrane Transport Proteins - metabolism, Gene Expression Regulation - physiology, Epithelial Cells - cytology, Electric Impedance, Dexamethasone, Cell Membrane Permeability - physiology, Butyric Acid, Blood-Brain Barrier - cytology, Tight Junctions - metabolism, gamma-Linolenic Acid

0 Comments

Editorial

01 February 2023 : Editorial  

Editorial: The XBB.1.5 (‘Kraken’) Subvariant of Omicron SARS-CoV-2 and its Rapid Global Spread

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.939580

Med Sci Monit 2023; 29:e939580

SARS-CoV-2/COVID-19

19 January 2023 : Clinical Research  

Evaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...

Med Sci Monit In Press; DOI: 10.12659/MSM.938892  

27 December 2022 : Clinical Research  

Effect of Physiotherapy to Correct Rounded Shoulder Posture in 30 Patients During the COVID-19 Pandemic in ...

Med Sci Monit 2022; 28:e938926

10 December 2022 : Clinical Research  

Retrospective Study to Identify Risk Factors for Severe Disease and Mortality Using the Modified Early Warn...

Med Sci Monit 2022; 28:e938647

In Press

06 Feb 2023 : Clinical Research  

Double C-Arm Digital Subtraction Angiography Guidance During Transjugular Intrahepatic Portosystemic Shunt ...

Med Sci Monit In Press; DOI: 10.12659/MSM.938912  

06 Feb 2023 : Review article  

Evolution of Hybrid Intelligence and Its Application in Evidence-Based Medicine: A Review

Med Sci Monit In Press; DOI: 10.12659/MSM.939366  

03 Feb 2023 : Clinical Research  

Treatment of Gingival Recession Defects with Pouch and Tunnel Technique Using Connective Tissue Graft and L...

Med Sci Monit In Press; DOI: 10.12659/MSM.938865  

02 Feb 2023 : Clinical Research  

Online Questionnaire-Based Study to Evaluate the Attitudes and Use of Rubber Dental Dams by Saudi Dental Pr...

Med Sci Monit In Press; DOI: 10.12659/MSM.938672  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750