Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

21 December 2009

Barrier functionality and transport machineries of human ECV304 cells

Jaleh BararABCEF, Mark GumbletonDEF, Masoud AsadiCF, Yadollah OmidiABCDEFG

Med Sci Monit 2010; 16(1): BR52-60 :: ID: 878321

Abstract

Background
The lack of a brain-derived endothelial cell-based model has led researchers to exploit non-brain endothelial/epithelial cells as in vitro models for blood-brain barrier (BBB) investigations. Of these, the endothelial-like human ECV304 cell line was initially characterized as endothelial cells and has been widely used as an in vitro BBB model. It was later shown to be T24 bladder carcinoma epithelial cells. To pursue its potential as a cell-based model for drug screening and transport machineries, ECV304 cells were evaluated for their barrier and plasma membrane characteristics.
Material and Method
ECV304 cells treated with some tight-junction modulators (e.g. cAMP elevators, butyric acid, dexamethasone, gamma-linolenic acid, and astrocytic factors) were examined for bioelectrical resistance, transcellular/paracellular permeability, and functionality expression of some key transporters.
Results
A significant (p<0.05) increase in trans-endothelial electrical resistance (TEER) was observed in ECV304 cells treated with astrocytic factors and cAMP elevators (i.e. to approximately 110Omega*cm2 vs. approximately 60 Omega*cm2 in the control). Such treatment also yielded high discrimination in the permeability coefficients of the transcellular marker propranolol (25x10-6 cm/sec) and the paracellular marker sucrose (11.3 x 10-6 cm/sec). Carrier-mediated transporters such as GLUT-1, system L, and P-glycoprotein (P-gp) as well as endocytic transport machineries (e.g. clathrin and caveolin) were found to be functionally expressed.
Conclusions
ECV304 cells failed to generate a discriminative tight barrier even in the presence of tight-junction modulators. Thus their implementation in drug permeability screening is not recommended. They display some important key carrier-mediated transport systems and can be considered as a useful cell-based in vitro model.

Keywords: Models, Biological, Membrane Transport Proteins - metabolism, Gene Expression Regulation - physiology, Epithelial Cells - cytology, Electric Impedance, Dexamethasone, Cell Membrane Permeability - physiology, Butyric Acid, Blood-Brain Barrier - cytology, Tight Junctions - metabolism, gamma-Linolenic Acid

Add Comment 0 Comments

Editorial

01 June 2024 : Editorial  

Editorial: Concerns as Highly Pathogenic Avian Influenza (HPAI) Virus of the H5N1 Subtype is Identified in Dairy Cows and Other Mammals

Dinah V. Parums

DOI: 10.12659/MSM.945315

Med Sci Monit 2024; 30:e945315

0:00

In Press

Clinical Research  

Anterior Plate-Supported Cannulated Screw Surgery for Ankle Arthrodesis: Clinical and Radiologic Results in...

Med Sci Monit In Press; DOI: 10.12659/MSM.944452  

Clinical Research  

Elevated Plasma Levels of Growth Arrest Specific 6 (Gas6) Protein in Severe Obesity: Implications for Adipo...

Med Sci Monit In Press; DOI: 10.12659/MSM.944462  

Database Analysis  

Systemic Immune-Inflammation Index (SII) as a Predictor of Short-Term Mortality Risk in Sepsis-Associated A...

Med Sci Monit In Press; DOI: 10.12659/MSM.943414  

Clinical Research  

Morphological Variations of the Nasopalatine Canal in the North Cyprus Population: A Cone Beam Computed Tom...

Med Sci Monit In Press; DOI: 10.12659/MSM.944868  

Most Viewed Current Articles

17 Jan 2024 : Review article   2,131,100

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research   1,555,090

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

16 May 2023 : Clinical Research   690,471

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

01 Jan 2022 : Editorial   50,389

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750