15 June 2017 : Laboratory Research
Effects of Connexin43 Overexpression on U251 Cell Growth, Migration, and Apoptosis
Jia Lu1BCE, Mingyue Yu2BCD, Zhen Lin3BCD, Su Lue3BCD, Hao Zhang3DF, Hang Zhao3DF, Yanyan Xu3DF, Haiyan Liu4ABCEG*DOI: 10.12659/MSM.905130
Med Sci Monit 2017; 23:2917-2923
Abstract
BACKGROUND: Glioblastoma multiforme (GBM) is a highly aggressive malignant brain tumor with a high incidence in adults. Connexin43 (Cx43) has general roles in tumorigenesis and is expressed in U251 glioma cells. Accordingly, the effects of Cx43 on the growth, migration, and apoptosis and the underlying mechanisms mediating Cx43-dependent migration and apoptosis were examined in U251 cells.
MATERIAL AND METHODS: A Cx43-overexpressing U251 cell line was generated to analyze the effects of Cx43 overexpression on cell growth, wound healing, and apoptosis-related protein expression after treatment with temozolomide.
RESULTS: The growth rate of U251 cells overexpressing Cx43 was significantly lower than that of parental wild-type cells, and cell morphology was considerably altered. The expression level of Bcl-2 was higher and the expression levels of Bax and caspase-3 were lower in cells overexpressing Cx43 than in wild-type cells. Additionally, the Bax/Bcl-2 ratio decreased.
CONCLUSIONS: Cx43 inhibited the growth of U251 cells, promoted morphological changes and migration, and inhibited apoptosis via a mitochondria-associated pathway.
Keywords: Connexin 43, Glioma
Editorial
01 July 2026 : Editorial
Editorial: The WHO Identifies Ebola Disease Due to Bundibugyo Virus as a Public Health Emergency of International Concern (PHEIC) as Vaccine Development Accelerates
DOI: 10.12659/MSM.954627
Med Sci Monit 2026; 32:e954627
In Press
Clinical Research
Body Weight and Insulin Resistance Indicators Among ChildrenMed Sci Monit In Press; DOI: 10.12659/MSM.951434
Clinical Research
Comparison of Radiographic Cervical Sagittal Alignment Parameters in Patients With Nonspecific Neck Pain, D...Med Sci Monit In Press; DOI: 10.12659/MSM.952950
Clinical Research
Combined Fibrinogen and Urinary α1-Microglobulin as Predictors of Respiratory Tract Infection in Children w...Med Sci Monit In Press; DOI: 10.12659/MSM.951066
Database Analysis
Evaluation of Salivary Total Oxidant Status (TOS) and Total Antioxidant Status (TAS) in Orthodontic Patient...Med Sci Monit In Press; DOI: 10.12659/MSM.952052
Most Viewed Current Articles
17 Jan 2024 : Review article 14,175,576
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
13 Nov 2021 : Clinical Research 3,756,620
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
14 Dec 2022 : Clinical Research 2,465,966
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research 708,651
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387