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12 October 2019 : Laboratory Research  

Long Non-Coding RNA Plasmacytoma Variant Translocation 1 (PVT1) Enhances Proliferation, Migration, and Epithelial-Mesenchymal Transition (EMT) of Pituitary Adenoma Cells by Activating β-Catenin, c-Myc, and Cyclin D1 Expression

Yihua Zhang1BCDEF, Yang Tan1BCDF, Hao Wang1BCDF, Minhui Xu1BCDF, Lunshan Xu1ABCDEG*

DOI: 10.12659/MSM.917110

Med Sci Monit 2019; 25:7652-7659

Abstract

BACKGROUND: As a kind of benign tumor, pituitary adenomas have attracted increasing attention from researchers. The plasmacytoma variant translocation 1 (PVT1) is a molecule in the lncRNA family protein that has been proven to play critical roles in many cancers; however, no study has explored the special biological roles of PVT1 in pituitary adenoma.

MATERIAL AND METHODS: The qRT-PCR assay was conducted to evaluate PVT1 expressions in various cell lines and tissues. Loss of function assays were carried out to detect the influence of silenced PVT1 on the proliferation, migration, and epithelial-mesenchymal transition (EMT) of pituitary adenoma cells. Western blotting was used to identify correlation between β-catenin and PVT1.

RESULTS: The PVT1 expressions were significantly enhanced in tissues of pituitary adenoma and cancer cells. Cell migration and proliferation were inhibited when the PVT1 gene was knocked down. Knockdown of PVT1 repressed the migration and EMT of pituitary adenoma cells. The PVT1 downregulation obviously blocked Wnt/β-catenin signaling pathway activity. PVT1 aggravated progression of pituitary adenoma through initiating the Wnt/β-catenin signaling pathway.

CONCLUSIONS: PVT1 exerts an oncogenic role through activating Wnt/β-catenin signaling in pituitary adenoma cells. The present results may provide a potential therapeutic target or approach for treating pituitary adenomas.

Keywords: alpha Catenin, Cell Migration Assays, Genes, myc, Pituitary Neoplasms, Adenoma, Biomarkers, Tumor, Cell Movement, Cell Proliferation, Cyclin D1, Epithelial-mesenchymal transition, Gene Expression Regulation, Neoplastic, Proto-Oncogene Proteins c-myc, RNA, Long Noncoding, RNA, Small Interfering, Wnt Signaling Pathway, beta Catenin

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750