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11 February 2020 : Laboratory Research

[Retracted: 21 Jan 2021] Pleuromutilin Inhibits Proliferation and Migration of A2780 and Caov-3 Ovarian Carcinoma Cells and Growth of Mouse A2780 Tumor Xenografts by Down-Regulation of pFAK2

Bo Zhang1BCDE, Xiaoli Ma1CEF, Yuan Li2BCD, Sijing Li3CDE, Jiumei Cheng1ADEF*

DOI: 10.12659/MSM.920407

Med Sci Monit 2020; 26:e920407

Some cells or groups of cells in figures appear to be visible multiple times. Because the manuscript contains non-credible results this journal is retracting the above publication. Reference: Bo Zhang, Xiaoli Ma, Yuan Li, Sijing Li, Jiumei Cheng: Pleuromutilin Inhibits Proliferation and Migration of A2780 and Caov-3 Ovarian Carcinoma Cells and Growth of Mouse A2780 Tumor Xenografts by Down-Regulation of pFAK2. Med Sci Monit, 2020; 26: e920407. DOI: 10.12659/MSM.920704

Abstract

BACKGROUND: Pleuromutilin is a natural tricyclic, derived from the fungus, Pleurotus mutilus. This study aimed to investigate the effects of pleuromutilin on migration and proliferation of A2780 and Caov-3 human ovarian carcinoma cells and the growth of A2780 tumor xenografts in mice and the molecular mechanisms involved.

MATERIAL AND METHODS: A2780 and Caov-3 human ovarian carcinoma cells were cultured with and without 40, 160, and 200 μM of pleuromutilin. The Edu fluorescence assay, the wound-healing assay, and Matrigel were used to measure A2780 and Caov-3 cell proliferation, migration, invasion, and adhesion in vitro, respectively. Western blot measured protein levels of FAK, p-FAK, MMP-2, and MMP-9. A2780 cells were injected subcutaneously into mice to determine the effects of pleuromutilin on the growth of tumor xenografts.

RESULTS: Pleuromutilin significantly reduced A2780 and Caov-3 cell proliferation at 48 h in a dose-dependent manner (P<0.05), and at 200 μM, pleuromutilin reduced cell proliferation by 21.43% and 23.65%, respectively. Treatment of A2780 cells with pleuromutilin significantly reduced cell migration, invasion, and adhesion and the expression of p-FAK, MMP-2, and MMP-9 compared with untreated controls. In the mouse tumor xenograft model, treatment with pleuromutilin significantly reduced tumor size compared with the untreated group and inhibited tumor metastasis to the intestine, spleen, and peritoneal cavity.

CONCLUSIONS: In A2780 and Caov-3 human ovarian carcinoma cells, pleuromutilin inhibited cell proliferation, migration, invasion, and adhesion in a dose-dependent manner, and reduced tumor growth and metastases in a mouse A2780 cell tumor xenograft model.

Keywords: Retracted Publication

Retraction note

Med Sci Monit 2021; 27:e930778     https://medscimonit.com/abstract/index/idArt/930778
 
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Medical Science Monitor eISSN: 1643-3750
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