02 July 2004
Granulocyte-macrophage colony-stimulating factor (GM-CSF) in children with acute immune thrombocytopenic purpura
Elham A. Rizk, Wessam M El-Gendy, Hoda Hassab, Lamyaa El-MadboullyMed Sci Monit 2004; 10(7): CR330-335 :: ID: 11694
Abstract
Background:The purpose of our study was to assess the serum level of GM-CSF in children with acute ITP and its correlation with clinical parameters and with the level of platelet-associated immunoglobulins.Material/Methods:Thirty children with acute ITP were selected from the inpatient department at Alexandria University Children’s Hospital at El Shatby. Ten apparently healthy children served as controls. ITP Patients were divided according to platelet count into those with platelet count >20,000/ul and cases with platelet counts >20,000/ul and cases with platelet counts <20,000/ul. Serum GM-CSF was measured by the ELISA technique. Total IgG was measured by radial immunodiffusion plates, and PAIgG was detected directly by flow cytometry.
Results: Total IgG showed significant elevation in ITP patients as compared to controls (t=2.748, P<0.05), but no correlation was detected between platelet antibodies and total IgG (r=–0.140, P=0.460). 86% of the patients had elevated platelet-associated immunoglobulin (PAIgG). No correlation between PAIgG and platelet count was detected (r=–0.072, P=0.72). Serum GM-CSF was significantly higher in ITP patients than controls (t=3.757, P<0.05). An inverse correlation was found between serum GM-CSF and platelet count (r=–0.4643, P=0.010). There was positive correlation between serum GM-CSF and PAIgG (r=0.4224, P=0.020).
Conclusions: Our results suggest that GM-CSF may have a role in the pathogenesis of ITP through activation of the mononuclear phagocyte system, resulting in accelerated destruction and phagocytosis of antibody-coated platelets and decreased platelet count.
Keywords: Adolescent, Autoantibodies - immunology, Blood Platelets - immunology, Blood Platelets - metabolism, Case-Control Studies, Child, Child, Preschool, Contusions - epidemiology, Contusions - etiology, Granulocyte-Macrophage Colony-Stimulating Factor - blood, Immunoglobulin G - blood, Immunoglobulins - blood, Immunoglobulins - immunology, Platelet Count, Purpura, Thrombocytopenic, Idiopathic - blood, Purpura, Thrombocytopenic, Idiopathic - complications, Purpura, Thrombocytopenic, Idiopathic - immunology, Statistics, Adolescent, Autoantibodies - immunology, Blood Platelets - metabolism, Case-Control Studies, Child, Child, Preschool, Contusions - etiology, Granulocyte-Macrophage Colony-Stimulating Factor - blood, Immunoglobulin G - blood, Immunoglobulins - immunology, Platelet Count, Purpura, Thrombocytopenic, Idiopathic - immunology, Statistics as Topic
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
12 Mar 2024 : Clinical Research
Preoperative Blood Transfusion Requirements for Hemorrhoidal Severe Anemia: A Retrospective Study of 128 Pa...Med Sci Monit In Press; DOI: 10.12659/MSM.943126
12 Mar 2024 : Clinical Research
Tissue Inhibitors of Metalloproteinase 1 (TIMP-1) and 3 (TIMP-3) as New Markers of Acute Kidney Injury Afte...Med Sci Monit In Press; DOI: 10.12659/MSM.943500
12 Mar 2024 : Review article
Optimizing Behçet Uveitis Management: A Review of Personalized Immunosuppressive StrategiesMed Sci Monit In Press; DOI: 10.12659/MSM.943240
12 Mar 2024 : Clinical Research
Metabolomic Alterations in Methotrexate Treatment of Moderate-to-Severe PsoriasisMed Sci Monit In Press; DOI: 10.12659/MSM.943360
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952