01 May 1997
Use of ESR spectroscopy in the evaluation of two risk factors in diabetic patients
Cezary Watała, Jacek Golański, Tadeusz Pietrucha, Ryszard Gurbiel, Krzysztof GwodzińskiMed Sci Monit 1997; 3(3): MT410-416 :: ID: 501315
Abstract
Background: Elevated plasma fibrinogen levels which contribute to increases in whole blood viscosity and the augmented nonenzymatic glycosylation of hemoglobin underlying the reduced red blood cell deformability play important roles in the impaired blood fluid dynamics encountered in diabetes mellitus. The need for reliable monitoring and adequate determination of the physical parameters governing blood viscosity underlies the reason for studying the contributions of two risk factors in diabetes: plasma hyperfibrinogenaemia and red blood cell deformability.
Material/Methods: Using ESR spectroscopy, the viscosity of the solutions of fibrinogen and haemoglobin was determined based on the rotational mobility of two piperidine-1-oxyl spin labels: TEMPONE (4-oxo-2,2,6,6-tetramethylpiperidine-1-oxyl) and TEMPAMINE (4-amino-2,2,6,6-tetramethylpiperidine-1-oxyl).
Results: We demonstrated that: a/ certain physical parameters relevant to whole blood viscosity and fluid dynamics can be easily monitored in a routine laboratory practice with the use of ESR spectrometry; b/ various water-soluble piperidine-1-oxyl derivatives can serve this purpose; c/ the linear or quasi-linear relationships between the concentrations of either fibrinogen or haemoglobin and the relevant relative viscosity of the protein solution hold within relatively broad ranges of protein concentrations; d/ plasma viscosities correlated significantly with plasma fibrinogen levels but not with plasma albumin levels; e/ glycosylation of haemoglobin correlated very significantly with both the erythrocyte intracellular viscosity and the viscosity of haemoglobin solutions.
Conclusions: 1. Monitoring of plasma viscosity, intracellular red blood cell viscosity and the viscosities of haemoglobin solutions could be complementary determinations performed to evaluate the impaired blood fluid dynamics in diabetic state; 2. ESR spectroscopy appears to be a simple, reproducible and seful diagnostic tool for monitoring some properties of blood fluid dynamics.
Keywords: ESR spectroscopy, TEMPO derivatives, Diabetes Mellitus, Blood Viscosity, glycosylated haemoglobin, internal viscosity, erythrocyte deformability hyperfibrinogenaemia
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
08 Mar 2024 : Animal Research
Modification of Experimental Model of Necrotizing Enterocolitis (NEC) in Rat Pups by Single Exposure to Hyp...Med Sci Monit In Press; DOI: 10.12659/MSM.943443
18 Apr 2024 : Clinical Research
Comparative Analysis of Open and Closed Sphincterotomy for the Treatment of Chronic Anal Fissure: Safety an...Med Sci Monit In Press; DOI: 10.12659/MSM.944127
08 Mar 2024 : Laboratory Research
Evaluation of Retentive Strength of 50 Endodontically-Treated Single-Rooted Mandibular Second Premolars Res...Med Sci Monit In Press; DOI: 10.12659/MSM.944110
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952