01 March 2004
Factors raising intracellular calcium increase red blood cell heterogeneity in density and critical osmolality
Irene L. Lisovskaya, Julian M. Rozenberg, Vitaly M. Nesterenko, Alexandra A. SamokhinaMed Sci Monit 2004; 10(3): BR67-76 :: ID: 11607
Abstract
Background:In normal human blood, RBC volume (V), surface area (A) and hemoglobin content (H) exhibit Gaussian distributions with coefficients of variation (CV) of 10–15%. Strikingly narrower distributions are observed for their cell density (CV~0.5%) and filterability (CV~5–7%). This implies that V is highly correlated with H and A. We hypothesize that the RBC is able to adjust its volume to parameters H and A. It is tempting to speculate that intracellular free calcium (Cai) is a mediator in this process, acting as an activator of the Gardos channel. We tested this hypothesis by experimentally varying Cai and measuring changes in RBC density and filterability distributions.Material/Methods: Three different approaches were used to raise Cai: (i) RBCs were loaded with Ca2+ in the presence of the calcium ionophore A23187; (ii) RBCs were incubated with Ca2+ in the presence of 1.0 mM ortho-vanadate; and (iii) the calcium pump was switched off by ATP depletion of RBCs. The density distribution of RBCs was determined by a phthalate technique. The distributions in filterability were obtained using a kinetic filtrometer.Results: Whatever the approach used, the density and filterability distributions of treated RBCs broadened significantly in comparison with those of control cells.Conclusions: The results obtained suggest that (i) in vivo regulation of RBC volume is mediated by Cai and (ii) Cai probably depends on the A/V ratio, which determines the stress experienced by the RBC membrane in the circulation and, thereby, the calcium influx rate.
Keywords: Glucose - metabolism, Adenosine Triphosphate - metabolism, Calcimycin - pharmacology, Calcium - metabolism, Dose-Response Relationship, Drug, Erythrocytes - metabolism, Glucose - metabolism, Ionophores - pharmacology, Normal Distribution, Osmolar Concentration, Temperature, Time Factors, Vanadates - pharmacology
639 0
Editorial
01 November 2023 : Editorial
Editorial: Factors Driving New Variants of SARS-CoV-2, Immune Escape, and Resistance to Antiviral Treatments as the End of the COVID-19 Pandemic is DeclaredDOI: 10.12659/MSM.942960
Med Sci Monit 2023; 29:e942960
In Press
28 Nov 2023 : Clinical Research
Impact of Fluorodeoxyglucose-Positron Emission Tomography/Computed Tomography on Therapeutic Decisions and ...Med Sci Monit In Press; DOI: 10.12659/MSM.942122
28 Nov 2023 : Clinical Research
Long-Term Outcomes of Decompression and Grafting in Acute Pathological Proximal Femur Fractures in Children...Med Sci Monit In Press; DOI: 10.12659/MSM.943031
27 Nov 2023 : Clinical Research
Comparison of Outcomes from Emergency Admissions to a Major Trauma Center in Turkey of 1646 Elderly Patient...Med Sci Monit In Press; DOI: 10.12659/MSM.942916
27 Nov 2023 : Clinical Research
Evaluating Modified Ultrasound-Guided Serratus Anterior Plane Block for Enhanced Postoperative Recovery in ...Med Sci Monit In Press; DOI: 10.12659/MSM.942757
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292