01 August 2004
Growth hormone enhances gastric ulcer healing in ratsStefan Beckert, Nicole Class, Farshid Farrahi, Stephan Coerper
Med Sci Monit 2004; 10(8): BR255-258 :: ID: 11740
Background: Gastric ulcer healing requires the reconstitution of epithelial structures and underlying connective tissue through cellular proliferation, migration, and differentiation. The systemic application of growth hormone (GH) has shown anabolic effects in postoperative and burn therapy by increasing protein synthesis and attenuating protein catabolism. There is also evidence that GH stimulates cell proliferation and differentiation. In this study we evaluated the impact of GH on gastric ulcer healing.
Material/Methods: Gastric ulcers were induced with a cryoprobe in male Wistar rats (285±11 g). The first group of rodents (n=10) received a daily subcutaneous dose of 2.5 mg/kg growth hormone for seven days. The second group (n=10) received only vehicle. After 7 days, ulcer size was determined photoplanimetrically. Cell proliferation and new vessel growth in the ulcer margin were evaluated by quantitative immunohistochemical staining for proliferating cell nuclear antigen (PCNA) and smooth muscle actin (SMA), respectively.
Results: The systemic application of GH caused a significant increase in body weight (332±7 g vs. 289±13 g; p=0.007). Ulcer size was also reduced significantly compared with controls (5.6±0.8 mm2 vs. 9.9±1 mm2; p=0.005). Immunohistochemical analysis revealed a significant increase in cell proliferation (79.7±0.9% pos. cells vs. 64.7±1.9% pos. cells; p=0.0001) as shown by PCNA expression, and a significant increase in new vessel growth as demonstrated by SMA expression (1762±124 cells/mm2 vs. 1067±77 cells/mm2; p=0.0001).
Conclusions: Growth hormone accelerates gastric ulcer healing by stimulating cell proliferation and angiogenesis.
Keywords: Cell Division - drug effects, Gastric Mucosa - drug effects, Gastric Mucosa - pathology, Growth Hormone - pharmacology, Neovascularization, Pathologic - drug therapy, Rats, Wistar, Stomach Ulcer - drug therapy, Wound Healing - drug effects, Cell Division - drug effects, Gastric Mucosa - pathology, Growth Hormone - pharmacology, Neovascularization, Pathologic - drug therapy, Rats, Wistar, Stomach Ulcer - drug therapy, Wound Healing - drug effects
01 June 2023 : EditorialEditorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic Preparedness
Med Sci Monit 2023; 29:e941209
01 Jun 2023 : Clinical ResearchRoot Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam ...
Med Sci Monit In Press; DOI: 10.12659/MSM.940472
01 Jun 2023 : Clinical ResearchInternal Orifice Alloy Closure: A New Procedure for Treatment of Perianal Fistulizing Crohn’s Disease
Med Sci Monit In Press; DOI: 10.12659/MSM.940873
01 Jun 2023 : Clinical ResearchEffectiveness of Needle Aspiration versus Surgical Excision for Symptomatic Synovial Cysts of the Hip: A Si...
Med Sci Monit In Press; DOI: 10.12659/MSM.940187
01 Jun 2023 : Clinical ResearchInfluence of Insulin Resistance on Diabetes Nephropathy
Med Sci Monit In Press; DOI: 10.12659/MSM.939482
Most Viewed Current Articles
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : EditorialEditorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...
Med Sci Monit 2022; 28:e935952